SDHB Antibody

Images

 
Western Blot: SDHB Antibody [NBP1-54154] - analysis of SDHB in porcine whole skeletal muscle lysate using anti-SDHB antibody. The primary antibody was used at a dilution of 1:2000 and incubated for 18 hours at 4C. Image ...read more
Immunocytochemistry/ Immunofluorescence: SDHB Antibody [NBP1-54154] - ICC staining of SDHB in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: SDHB Antibody [NBP1-54154] - IHC staining of SDHB in mouse bladder.
Western Blot: SDHB Antibody [NBP1-54154] - Detection of SDHB in mouse kidney lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.14 mg/ml

SDHB Antibody Summary

Immunogen
A genomic peptide made to an N-terminal region of the human SDHB protein (within residues 1-150). [Swiss-Prot P21912]
Localization
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Marker
Mitochondria Marker
Predicted Species
Rat (92%), Porcine (92%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDHB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:7000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This SDHB antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~31 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
Jurkat Whole Cell Lysate
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-54154 in the following applications:

Publications
Read Publications using
NBP1-54154 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse. Immunogen sequence has 92% identity to rat, cow and pig, 85% identity to Xenopus and 84% identity to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.14 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for SDHB Antibody

  • EC 1.3.5.1
  • FLJ92337
  • IP
  • Iron-sulfur subunit of complex II
  • PGL4
  • SDH
  • SDH1
  • SDH2
  • SDHIP
  • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
  • succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Background

SDHB [Succinate dehydrogenase (ubiquinone) iron-sulfur subunit, mitochondrial] is an iron-sulfur protein subunit of succinate dehydrogenase (SDH) which is involved in mitochondrial electron transport chain's complex II and is accountable for electrons transfer from succinate to ubiquinone/coenzyme Q. The complex II is a key respiratory enzyme which links Krebs cycle and electron transport chain, and is composed of four subunits: the flavoprotein SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD as well as additional groups such as iron-sulphur centres and ubiquinone. SDH complex functions as a tumour suppressor, and loss of any of the subunit proteins leads to destabilization of the complex that follows carcinogenesis. Defects in SDHB have also been linked to susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS), and Cowden-like syndrome (CWDLS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SDHB Antibody (NBP1-54154)(3)

Review for SDHB Antibody (NBP1-54154) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-54154:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot SDHB NBP1-54154
Enlarge
3
reviewed by:
Anonymous
WB Other 11/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate or Purified Mitochondria
SpeciesOther
LotA-1

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25000

Details

Detection NotesLicor Odyssey

Comments

CommentsAntibody works great for purified mitochondria, but struggles with whole skeletal muscle lysate. Also has some non-specific binding (70 kDa and 45 kDa). That's why we gave it a 3 star rating, but it does identify SDHB at 27 kDa.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SDHB Antibody (NBP1-54154) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-54154AF350
Alexa Fluor 405 NBP1-54154AF405
Alexa Fluor 488 NBP1-54154AF488
Alexa Fluor 532 NBP1-54154AF532
Alexa Fluor 594 NBP1-54154AF594
Alexa Fluor 647 NBP1-54154AF647
Alexa Fluor 700 NBP1-54154AF700
Alexa Fluor 750 NBP1-54154AF750
Biotin NBP1-54154B
DyLight 350 NBP1-54154UV
DyLight 405 NBP1-54154V
DyLight 488 NBP1-54154G
DyLight 550 NBP1-54154R
DyLight 594 NBP1-54154DL594
DyLight 650 NBP1-54154C
DyLight 680 NBP1-54154FR
DyLight 755 NBP1-54154IR
FITC NBP1-54154F
HRP NBP1-54154H
Janelia Fluor 549 NBP1-54154JF549
Janelia Fluor 646 NBP1-54154JF646

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Bioinformatics Tool for SDHB Antibody (NBP1-54154)

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Blogs on SDHB.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

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Recent Reviews

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1
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Anonymous
11/17/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol SDHB
Entrez
Uniprot