SDC3 Antibody [Allophycocyanin]

• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

SDC3 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Syndecan-3
  Gln48-Lys383
  Accession # O75056
• Specificity: Detects human Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Syndecan-3 is observed and less than 1% cross-reactivity with recombinant mouse Syndecan-4 and recombinant human Syndecan-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SDC3
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1‑3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1‑3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1‑86 or deletion of aa 1‑96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120‑150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta , it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11‑13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
4. ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDC3