SDC3 Antibody [Unconjugated]

Syndecan‑3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Syndecan‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2734) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SDC3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
  Ala45-Glu384
  Accession # Q64519
• Specificity: Detects mouse Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Syndecan-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SDC3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDC3 Antibody [Unconjugated]

• KIAA0468
• N-Syndecan
• SDC3
• SDCN
• SYND3syndecan proteoglycan 3
• syndecan 3 (N-syndecan)
• syndecan 3
• syndecan neural type
• Syndecan3
• Syndecan-3

Background

Mouse Syndecan-3 (also known as N-Syndecan) is a variably glycosylated type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 442 amino acid (aa) precursor with a 45 aa signal sequence, a 339 aa extracellular domain (ECD), a 25 aa TM segment and a 33 aa cytoplasmic region. It presumably exists as a dimer. Heparan sulfate modification of the ECD yields a native molecular weight that exceeds 200 kDa. Soluble forms generated by proteolytic processing are suggested to exist. Syndecan-3 is a musculoskeletal and neuronal molecule which mediates neurite outgrowth and myoblast differentiation. Mouse Syndecan-3 ECD shares 95% and 81% aa sequence identity with rat and human Syndecan-3 ECD, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDC3 Antibody (AF2734)(2)

Publications using AF2734

• Thomas Welte, Junhua Mai, Zhe Zhang, Shaohui Tian, Guodong Zhang, Yitian Xu et al. A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells iScience 2021-11-19 [PMID: 34825135]
• Hosono-Fukao T, Ohtake-Niimi S, Hoshino H, Britschgi M, Akatsu H, Hossain MM, Nishitsuji K, van Kuppevelt TH, Kimata K, Michikawa M, Wyss-Coray T, Uchimura K Heparan sulfate subdomains that are degraded by Sulf accumulate in cerebral amyloid beta plaques of Alzheimer's disease: evidence from mouse models and patients. Am. J. Pathol., 2012-03-17;180(5):2056-67. 2012-03-17 [PMID: 22429964] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: SDC3
• Uniprot:
  • Q64519()