SDC3 Antibody (312607)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
312607
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SDC3 Antibody (312607) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
Ala45-Glu384
Accession # Q64519
Specificity
Detects mouse Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) Syndecan-1, rmSyndecan-4, or recombinant human Syndecan-3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
SDC3
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2734 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDC3 Antibody (312607)

  • KIAA0468
  • N-Syndecan
  • SDC3
  • SDCN
  • SYND3syndecan proteoglycan 3
  • syndecan 3 (N-syndecan)
  • syndecan 3
  • syndecan neural type
  • Syndecan3
  • Syndecan-3

Background

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of mouse Syndecan-3 shares 95%, 83%, 82% and 81% aa identity with of rat, human, canine and bovine Syndecan-3, respectively. Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 - 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 - 13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).
  1. Xian, X. et al. (2010) Cell Tissue Res. 339:31.
  2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
  3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
  4. ENTREZ protein Accession # Q64519.
  5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
  7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
  8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
  9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
  10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
  11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
  12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
  13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
  14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SDC3 Antibody (MAB2734)(3)

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Bioinformatics

Gene Symbol SDC3
Uniprot