SDC3 Antibody (312607)

• Reactivity: Mu
• Applications: WB
• Clone: 312607
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

SDC3 Antibody (312607) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
  Ala45-Glu384
  Accession # Q64519
• Specificity: Detects mouse Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) Syndecan-1, rmSyndecan-4, or recombinant human Syndecan-3 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SDC3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDC3 Antibody (312607)

• KIAA0468
• N-Syndecan
• SDC3
• SDCN
• SYND3syndecan proteoglycan 3
• syndecan 3 (N-syndecan)
• syndecan 3
• syndecan neural type
• Syndecan3
• Syndecan-3

Background

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of mouse Syndecan-3 shares 95%, 83%, 82% and 81% aa identity with of rat, human, canine and bovine Syndecan-3, respectively. Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 - 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta , it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 - 13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

1. Xian, X. et al. (2010) Cell Tissue Res. 339:31.
2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
4. ENTREZ protein Accession # Q64519.
5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDC3 Antibody (MAB2734)(3)

Publications using MAB2734

• Kowalewska P, Patrick A, Fox-Robichaud A Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation. PLoS ONE, 2014-09-03;9(9):e104537. 2014-09-03 [PMID: 25184228] (IHC-Fr, Mouse)
• Floer M, Gotte M, Wild MK, Heidemann J, Gassar ES, Domschke W, Kiesel L, Luegering A, Kucharzik T Enoxaparin improves the course of dextran sodium sulfate-induced colitis in syndecan-1-deficient mice. Am. J. Pathol., 2009-12-11;176(1):146-57. 2009-12-11 [PMID: 20008145] (IHC-Fr, Mouse)
• Kharabi Masouleh B, ten Dam GB, Wild MK, Seelige R, van der Vlag J, Rops AL, Echtermeyer FG, Vestweber D, van Kuppevelt TH, Kiesel L, Gotte M Role of the heparan sulfate proteoglycan syndecan-1 (CD138) in delayed-type hypersensitivity. J. Immunol., 2009-04-15;182(8):4985-93. 2009-04-15 [PMID: 19342678] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: SDC3
• Uniprot:
  • Q64519()