Scaffold attachment factor B2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Scaffold attachment factor B2 Antibody [NBP2-98007] - Immunofluorescence staining of Scaffold attachment factor B2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Scaffold attachment factor B2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus.

Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody [NBP2-98007] - Immunochemical staining of human Scaffold attachment factor B2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody [NBP2-98007] - Immunochemical staining of human Scaffold attachment factor B2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Scaffold attachment factor B2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Scaffold attachment factor B2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAFB2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Scaffold attachment factor B2 Antibody - BSA Free

• KIAA0138SAF-B2
• scaffold attachment factor B2

Background

Scaffold attachment factor B2 (SAFB2) is a chromatin associated protein that interacts with DNA elements termed S/MARs (scaffold or matrix attachment regions) but is not part of the nuclear matrix. SAFB2 is structurally and functionally similar to SAFB1 and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. SAFB2 has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAFB2