Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca‑1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Sca-1/Ly6 Antibody
lymphocyte antigen 6 family member G6D
This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
Spangrude, G.I. et al. (1988) Science 241:58.
Spangrude, G.I. et al. (1993) Blood 82:3327.
Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
Yamamoto, Y. et al. (1996) Blood 88:445.
Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
Welm, B.E. et al. (2002) Dev. Biol. 245:42.
Codias, E.K. et al. (1990) J. Immunol. 144:2197.
Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
Codias, E.K. et al. (1990) J. Immunol. 145:1407.
Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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