Sca-1/Ly6 Antibody

Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca‑1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Sca-1/Ly6 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
  Leu27-Gly119
  Accession # CAA28351
• Specificity: Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ly6a
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sca-1/Ly6 Antibody

• C6orf23
• G6D
• Ly6
• Ly-6
• LY6-D
• LY6G6D
• lymphocyte antigen 6 family member G6D
• MEGT1
• NG25
• Sca1
• Sca-1

Background

This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

1. Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
2. Spangrude, G.I. et al. (1988) Science 241:58.
3. Spangrude, G.I. et al. (1993) Blood 82:3327.
4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
6. Yamamoto, Y. et al. (1996) Blood 88:445.
7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sca-1/Ly6 Antibody (AF1226)(21)

Publications using AF1226

• EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (IHC, Mouse)
• H Cui, Z Li, S Chen, X Li, D Chen, J Wang, Z Li, W Hao, F Zhong, K Zhang, Z Zheng, Z Zhan, H Liu CXCL12/CXCR4-Rac1-mediated migration of osteogenic precursor cells contributes to pathological new bone formation in ankylosing spondylitis Science Advances, 2022-04-06;8(14):eabl8054. 2022-04-06 [PMID: 35385310] (IHC, Mouse, Transgenic Mouse)
• F Kleefeldt, B Upcin, H Bömmel, C Schulz, G Eckner, J Allmanritt, J Bauer, B Braunger, U Rueckschlo, S Ergün Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis Cell Death & Disease, 2022-03-09;13(3):220. 2022-03-09 [PMID: 35264563] (ICC, Mouse)
• ES Kim, BH Yoon, SM Lee, M Choi, EH Kim, BW Lee, SY Kim, CG Pack, YH Sung, IJ Baek, CH Jung, TB Kim, JY Jeong, CH Ha Fecal microbiota transplantation ameliorates atherosclerosis in mice with C1q/TNF-related protein 9 genetic deficiency Experimental & Molecular Medicine, 2022-02-03;0(0):. 2022-02-03 [PMID: 35115674] (IHC, Mouse)
• P Pagella, C Nombela-Ar, TA Mitsiadis Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth International Journal of Molecular Sciences, 2021-03-16;22(6):. 2021-03-16 [PMID: 33809663] (IHC, Mouse)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)
• L Golle, HU Gerth, K Beul, B Heitplatz, P Barth, M Fobker, H Pavenstädt, GS Di Marco, M Brand Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms Sci Rep, 2017-08-25;7(1):9444. 2017-08-25 [PMID: 28842629] (Flow Cytometry, Rat)
• E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress J Am Heart Assoc, 2017-07-14;6(7):. 2017-07-14 [PMID: 28710180] (ICC, Mouse)
• JM Daniel, J Dutzmann, H Brunsch, J Bauersachs, R Braun-Dull, DG Sedding Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties Int. J. Cardiol., 2017-03-14;0(0):. 2017-03-14 [PMID: 28433555] (IHC, Mouse)
• Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K Pluripotent stem cells derived from mouse and human white mature adipocytes. Stem Cells Transl Med, 2014-01-06;3(2):161-71. 2014-01-06 [PMID: 24396033] (ICC, Mouse)
• Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-12-12;504(7479):277-81. 2013-12-12 [PMID: 24336287] (IHC-Fr, Mouse)
• Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy. Stem Cells Dev, 2013-08-30;22(24):3214-25. 2013-08-30 [PMID: 23895415] (IHC-P, Mouse)
• Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors. Bone, 2011-06-29;49(4):693-700. 2011-06-29 [PMID: 21741502] (IHC-Fr, Mouse)
• Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production. J. Biol. Chem., 2011-07-06;286(36):31409-17. 2011-07-06 [PMID: 21733845] (IHC-Fr, Mouse)
• Smith BH, Gazda LS, Conn BL Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties. Cancer Res., 2011-01-24;71(3):716-24. 2011-01-24 [PMID: 21266363] (IHC, Mouse)
• Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Luscher TF, Hunziker L, Zulewski H, Eriksson U Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis. Am. J. Respir. Crit. Care Med., 2009-02-20;179(10):939-49. 2009-02-20 [PMID: 19234103] (Flow Cytometry, Mouse)
• Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration. Nat. Genet., 2008-06-08;40(7):862-70. 2008-06-08 [PMID: 18536717] (ICC, Mouse)
• Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion. J. Immunol., 2008-03-15;180(6):3874-81. 2008-03-15 [PMID: 18322195] (IHC-Fr, Mouse)
• Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction. Biomaterials, 2007-11-26;29(7):797-808. 2007-11-26 [PMID: 18022690] (ICC, Rat)
• Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro. Proc. Natl. Acad. Sci. U.S.A., 2006-06-13;103(25):9530-5. 2006-06-13 [PMID: 16772384] (ICC, Mouse)
• Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T Identification of bronchioalveolar stem cells in normal lung and lung cancer. Cell, 2005-06-17;121(6):823-35. 2005-06-17 [PMID: 15960971] (ICC, Mouse)

Bioinformatics

• Gene Symbol: Ly6a
• Uniprot:
  • CAA28351()