Sca-1/Ly6 Antibody


Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca‑1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Sca-1/Ly6 Antibody Summary

Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
Accession # CAA28351
Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF1226 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sca-1/Ly6 Antibody

  • C6orf23
  • G6D
  • Ly6
  • Ly-6
  • LY6-D
  • LY6G6D
  • lymphocyte antigen 6 family member G6D
  • MEGT1
  • NG25
  • Sca1
  • Sca-1


This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

  1. Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
  2. Spangrude, G.I. et al. (1988) Science 241:58.
  3. Spangrude, G.I. et al. (1993) Blood 82:3327.
  4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
  5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
  6. Yamamoto, Y. et al. (1996) Blood 88:445.
  7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
  8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
  9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
  10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
  11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
  12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: Bind
Species: Hu
Applications: ICC/IF
Species: Hu
Applications: WB
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA, AP, PA, WB
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: BA
Species: Bv, Ce, Hu, I, Mu, Pl
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: IB, IHC, IHC-Fr, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Am, Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
Species: Ca, Ch, ChHa, Fe, Hu, Mu, Ma-Op, Po, Pm, Rt
Applications: ICC/IF, Simple Western, WB
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC/IF

Publications for Sca-1/Ly6 Antibody (AF1226)(21)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.

Filter By Application
Flow Cytometry
All Applications
Filter By Species
Transgenic Mouse
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF1226 Applications Species
EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (IHC, Mouse) IHC Mouse
H Cui, Z Li, S Chen, X Li, D Chen, J Wang, Z Li, W Hao, F Zhong, K Zhang, Z Zheng, Z Zhan, H Liu CXCL12/CXCR4-Rac1-mediated migration of osteogenic precursor cells contributes to pathological new bone formation in ankylosing spondylitis Science Advances, 2022-04-06;8(14):eabl8054. 2022-04-06 [PMID: 35385310] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
F Kleefeldt, B Upcin, H Bömmel, C Schulz, G Eckner, J Allmanritt, J Bauer, B Braunger, U Rueckschlo, S Ergün Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis Cell Death & Disease, 2022-03-09;13(3):220. 2022-03-09 [PMID: 35264563] (ICC, Mouse) ICC Mouse
ES Kim, BH Yoon, SM Lee, M Choi, EH Kim, BW Lee, SY Kim, CG Pack, YH Sung, IJ Baek, CH Jung, TB Kim, JY Jeong, CH Ha Fecal microbiota transplantation ameliorates atherosclerosis in mice with C1q/TNF-related protein 9 genetic deficiency Experimental & Molecular Medicine, 2022-02-03;0(0):. 2022-02-03 [PMID: 35115674] (IHC, Mouse) IHC Mouse
P Pagella, C Nombela-Ar, TA Mitsiadis Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth International Journal of Molecular Sciences, 2021-03-16;22(6):. 2021-03-16 [PMID: 33809663] (IHC, Mouse) IHC Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse) IHC Mouse
L Golle, HU Gerth, K Beul, B Heitplatz, P Barth, M Fobker, H Pavenstädt, GS Di Marco, M Brand Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms Sci Rep, 2017-08-25;7(1):9444. 2017-08-25 [PMID: 28842629] (Flow Cytometry, Rat) Flow Cytometry Rat
E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress J Am Heart Assoc, 2017-07-14;6(7):. 2017-07-14 [PMID: 28710180] (ICC, Mouse) ICC Mouse
JM Daniel, J Dutzmann, H Brunsch, J Bauersachs, R Braun-Dull, DG Sedding Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties Int. J. Cardiol., 2017-03-14;0(0):. 2017-03-14 [PMID: 28433555] (IHC, Mouse) IHC Mouse
Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K Pluripotent stem cells derived from mouse and human white mature adipocytes. Stem Cells Transl Med, 2014-01-06;3(2):161-71. 2014-01-06 [PMID: 24396033] (ICC, Mouse) ICC Mouse
Show All 21 Publications.

Reviews for Sca-1/Ly6 Antibody (AF1226) (0)

There are no reviews for Sca-1/Ly6 Antibody (AF1226). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sca-1/Ly6 Antibody (AF1226) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Sca-1/Ly6 Products

Array AF1226

Bioinformatics Tool for Sca-1/Ly6 Antibody (AF1226)

Discover related pathways, diseases and genes to Sca-1/Ly6 Antibody (AF1226). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sca-1/Ly6 Antibody (AF1226)

Discover more about diseases related to Sca-1/Ly6 Antibody (AF1226).

Pathways for Sca-1/Ly6 Antibody (AF1226)

View related products by pathway.

Blogs on Sca-1/Ly6

There are no specific blogs for Sca-1/Ly6, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Sca-1/Ly6 Antibody and receive a gift card or discount.


Gene Symbol Ly6a