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LY6G6D Products By Gene Name

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AF1226

Sca-1/Ly6 Antibody [Unconjugated]

(31 Publications)

100 μg

AF1226

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Sca1, a marker of mammary stem/progenitor cells, is increased in DNIIR and Wnt5a-/- glands. mRNA and protein were extracted from several wild type (WT) and MT-DNIIR (DNIIR) mice. (a) Sca1 mRNA levels were determined by semi-quantitative RT-PCR and (b) protein levels were determined by western blot. Sca1 levels were increased in DNIIR glands relative to controls. (c) Sca1 protein levels were determined in WT and Wnt5a null (-/-) glands using western blot analysis. Sca1 levels were increased in Wnt5a-null tissue relative to controls. 18S and beta -Tubulin ( beta -Tub) were used as normalisation controls. DNIIR = dominant-negative transforming growth factor-beta type II receptor; RT-PCR = reverse transcripase polymerase chain reaction; Wnt = Wingless-related protein family. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19344510), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ectopic expression of Wnt5a results in low expression of K6 and K14.Expression of molecular markers for basal and luminal progenitors in MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mammary glands was compared by western blot. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Each lane contains protein isolated from a separate mouse. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Wnt5a expressing tumors demonstrate a decrease in markers of the basal tumor subtype.(A) Western blot using protein lysates isolated from the epithelium of MMTV-Wnt1 and MMTV-1;MMTV-Wnt5a mammary tumors. The expression of molecular markers of basal and luminal tumor subtypes were compared. Keratin 6 and Keratin 5 were strongly down-regulated in Wnt5a expressing tumors. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (B–D) Immunostaining for K6. Sections from MMTV-Wnt1 (B) and MMTV-1;MMTV-Wnt5a (C) tumors were stained with anti-Keratin 6 antibody using immunofluorescence (K6 = green; nuclei = blue). The percentage of cells expressing K6 was determined and graphed (D). Values are means +/− standard error (n = 6 MMTV-Wnt1, 3 fields per tumor; n = 5 MMTV-Wnt1;MMTV-Wnt5a, 3 fields per tumor). MMTV-Wnt1;MMTV-Wnt5a tumors demonstrated a significant decrease in K6-expressing cells as measured by T-test (* = p<0.05). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sali spheres contain stem cells.(A) Sca-1 is present in the mouse submandibular gland on endothelial cells as well as on excretory and striated duct cells (D), and was clearly present at the onset of cultivation (D0) whereas acinar cells (AC) did not express Sca-1. At day 3 and later time-points, nearly all cells at the periphery of the Sali spheres showed high Sca-1 expression which decreased in time (D10). (B) Approximately 52.0+/−3.1% (Mean+/−STDEV) of cells in D3 cultured spheres expressed Sca-1, as quantified by flow cytometry. (C) c-Kit is only expressed by excretory duct cells (Tissue). Sali spheres showed similar c-Kit staining patterns as for Sca-1. (D) Most nuclei in excretory duct compartments and few nuclei in striated duct cells showed Musashi-1 presence (Tissue). Whereas Musashi-1 was still present in the nuclei of some cells at the onset of culturing (D0-arrow), most cells showed cytoplasmic localization which diminished in time (D3–5–10). Cells were visualized with DAPI (blue). Scale bar = 50 µm, inset = 20 µm. D = duct cells, AC = acinar cell, D0–3–5–10 represent days in culture. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/18446241), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1226U-100UG
Alexa Fluor 405	AF1226V-100UG
Alexa Fluor 488	AF1226G-100UG
Alexa Fluor 532	AF1226X-100UG
Alexa Fluor 594	AF1226T-100UG
Alexa Fluor 647	AF1226R-100UG
Alexa Fluor 700	AF1226N-100UG
Alexa Fluor 750	AF1226S-100UG
Alexa Fluor Plus 405	AF1226AFP405-100UG
Alexa Fluor Plus 488	AF1226AFP488-100UG
Alexa Fluor Plus 555	AF1226AFP555-100UG
Alexa Fluor Plus 594	AF1226AFP594-100UG
Alexa Fluor Plus 647	AF1226AFP647-100UG
Alexa Fluor Plus 680	AF1226AFP680-100UG
Allophycocyanin	AF1226A
Biotin	BAF1226
CoraFluor 1	AF1226CL1
DyLight 350	AF1226D
DyLight 405	AF1226E
DyLight 488	AF1226K
DyLight 550	AF1226L
DyLight 594	AF1226M
DyLight 650	AF1226W
DyLight 680	AF1226Y
DyLight 755	AF1226Z
FITC	AF1226F
HRP	AF1226H
Janelia Fluor 525	AF1226JF525
Janelia Fluor 549	AF1226I
Janelia Fluor 646	AF1226J
Janelia Fluor 669	AF1226JF669
PE	AF1226P
PerCP	AF1226C
mFluor Violet 450 SE	AF1226MFV450
mFluor Violet 500 SE	AF1226MFV500
mFluor Violet 610 SE	AF1226MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1226-SP

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NBP3-27164

LY6G6D Antibody (3D6E10) - BSA Free

0.1 ml

NBP3-27164

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against HEK293-6e (1) and Gr-1/Ly-6G (AA: 20-104)-hIgGFc transfected HEK293-6e (2) cell lysate.

ELISA: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against human Gr-1/Ly-6G (AA: 20-104) recombinant protein. (Expected MW is 21.4 kDa)

Immunocytochemistry/Immunofluorescence: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis of Hela cells using Gr-1/Ly-6G mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-27164-0.025ml

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FAB1037F

Gr-1/Ly-6G Antibody (RB6-8C5) [Fluorescein]

(5 Publications)

100 Tests

FAB1037F

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # <a class=

FAB1217A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) or (B) Rat IgG_2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins." alt="Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) or (B) Rat IgG_2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Fluorescein

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1037U-100UG
Alexa Fluor 405	FAB1037V-100UG
Alexa Fluor 488	FAB1037G-100UG
Alexa Fluor 532	FAB1037X
Alexa Fluor 594	FAB1037T-100UG
Alexa Fluor 647	FAB1037R-100UG
Alexa Fluor 750	FAB1037S-100UG
Alexa Fluor Plus 405	FAB1037AFP405-100UG
Alexa Fluor Plus 488	FAB1037AFP488-100UG
Alexa Fluor Plus 555	FAB1037AFP555-100UG
Alexa Fluor Plus 594	FAB1037AFP594-100UG
Alexa Fluor Plus 647	FAB1037AFP647-100UG
Alexa Fluor Plus 680	FAB1037AFP680-100UG
Allophycocyanin	FAB1037A-025
Allophycocyanin	FAB1037A-100
Allophycocyanin/Cy7	FAB1037APCCY7
Biotin	BAM1037
CoraFluor 1	FAB1037CL1
DyLight 350	FAB1037D
DyLight 405	FAB1037E
DyLight 488	FAB1037K
DyLight 550	FAB1037L
DyLight 594	FAB1037M
DyLight 650	FAB1037W
DyLight 680	FAB1037Y
DyLight 755	FAB1037Z
Fluorescein	FAB1037F-025
HRP	FAB1037H
Janelia Fluor 525	FAB1037JF525
Janelia Fluor 549	FAB1037I
Janelia Fluor 646	FAB1037J
Janelia Fluor 669	FAB1037JF669
PE/Atto594	FAB1037PEATT594
PE/Cy5.5	FAB1037PECY55
PE/Cy7	FAB1037PECY7
Phycoerythrin	FAB1037P-025
Phycoerythrin	FAB1037P-100
mFluor Violet 450 SE	FAB1037MFV450
mFluor Violet 500 SE	FAB1037MFV500
mFluor Violet 610 SE	FAB1037MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1037V-100UG
Carrier Free	FAB1037P-100
Carrier Free	FAB1037P-025
Carrier Free	FAB1037F-025
Carrier Free	FAB1037A-100
Carrier Free	FAB1037A-025
Carrier Free	FAB1037U-100UG
Carrier Free	FAB1037T-100UG
Carrier Free	FAB1037S-100UG
Carrier Free	FAB1037R-100UG
Carrier Free	FAB1037G-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

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Species Glossary

LY6G6D Products By Gene Name

Sca-1/Ly6 Antibody [Unconjugated]

LY6G6D Antibody (3D6E10) - BSA Free

Gr-1/Ly-6G Antibody (RB6-8C5) [Fluorescein]