Sca-1/Ly6 Antibody [Biotin]

Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Sca-1/Ly6 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1 C-terminally truncated Ly-6E allele
  Leu27-Gly119
  Accession # CAA28351
• Specificity: Detects Sca‑1/Ly6 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ly6a
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sca-1/Ly6 Antibody [Biotin]

• Ly6
• Ly-6
• Ly-6A.2
• Ly-6A/E
• Ly-6E.1
• lymphocyte antigen 6 complex, locus A
• lymphocyte antigen 6A-2/6E-1
• Sca1
• Sca-1
• Stem cell antigen 1
• TAP
• T-cell-activating protein

Background

This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

1. Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
2. Spangrude, G.I. et al. (1988) Science 241:58.
3. Spangrude, G.I. et al. (1993) Blood 82:3327.
4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
6. Yamamoto, Y. et al. (1996) Blood 88:445.
7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ly6a
• Uniprot:
  • CAA28351()