Sca-1/Ly6 Antibodies

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />