SC35 Antibody - BSA Free

Western Blot: SC35 Antibody [NBP3-12974] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SC35 antibody (NBP3-12974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: SC35 Antibody [NBP3-12974] - SRSF2 antibody detects SRSF2 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. SRSF2 antibody (NBP3-12974) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: SC35 Antibody [NBP3-12974] - SRSF2 antibody detects SRSF2 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SRSF2 antibody (NBP3-12974) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SC35 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SC35. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRSF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SC35 Antibody - BSA Free

• PR264
• SC35
• SC-35
• serine/arginine-rich splicing factor 2
• SFRS2
• SFRS2A
• splicing component, 35 kDa
• splicing factor SC35
• splicing factor, arginine/serine-rich 2
• SR splicing factor 2
• SRp30b

Background

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRSF2