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SRp55 Antibody

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Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.
Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.
Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified ...read more
Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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SRp55 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to this antibody using the numbering given in entry NP_006266.2 (GeneID 6431).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRSF6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
SRp55 Lysate (NBP2-65826)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP2-04142 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SRp55 Antibody

  • B52
  • FLJ08061
  • pre-mRNA splicing factor SRP55
  • Pre-mRNA-splicing factor SRP55
  • serine/arginine-rich splicing factor 6
  • SFRS6
  • Splicing factor, arginine/serine-rich 6MGC5045
  • splicing factor, arginine/serine-rich, 55 kDa
  • SR splicing factor 6
  • SRP55arginine/serine-rich splicing factor 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: SF2 Antibody [NBP2-20324] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse duodenum. SF2 antibody dilution: 1:500.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
gamma-Synuclein
Antibody (JM90-32)
NBP2-66861
Western Blot: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of gamma Synuclein on rat kidney tissue lysate using anti-gamma Synuclein antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Staining gamma Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of paraffin-embedded rat brain tissue using anti-gamma Synuclein antibody. Counter stained with hematoxylin.
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Secretin R Antibody
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Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human Secretin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6387) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Secretin R delta 3/4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB
ARSF Antibody
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Western Blot: ARSF Antibody [NBP1-86191] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: ARSF Antibody [NBP1-86191] - Staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: ARSF Antibody [NBP1-86191] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: ICC/IF
Proteasome 19S S5A
Antibody
NBP1-90821
Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP1-90821] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Proteasome 19S S5A Antibody [NBP1-90821] - Staining in human testis and pancreas tissues using anti-PSMD4 antibody. Corresponding PSMD4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Proteasome 19S S5A Antibody [NBP1-90821] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
RNPS1 Antibody
NBP1-92343
Western Blot: RNPS1 Antibody [NBP1-92343] - Analysis in human cell line RT-4
Immunocytochemistry/Immunofluorescence: RNPS1 Antibody [NBP1-92343] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RNPS1 Antibody [NBP1-92343] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SFRS5 Antibody
NBP1-92381
Western Blot: SFRS5 Antibody [NBP1-92381] - Analysis in human cell line HeLa.
Immunocytochemistry/Immunofluorescence: SFRS5 Antibody [NBP1-92381] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.
Immunohistochemistry-Paraffin: SFRS5 Antibody [NBP1-92381] - Staining in human fallopian tube and pancreas tissues. Corresponding SRSF5 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
SC35 Antibody
(SC-35)
NB100-1774
Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated on SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:100; 2. Antibody dilution 1:250; 3. Antibody dilution 1:500; 4. Antibody dilution 1:1,000
Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing Factor Clone: SC-35 diluted to 1:2,000. The antibody was developed using Goat Anti-Mouse IgG, FITC-conjugate.
(5 Publications)
Species: Hu, Rt, Am, Dr
Applications: WB, EM, ELISA, ICC/IF, IP
SFRS4 Antibody
NBP2-04144
Western Blot: SFRS4 Antibody [NBP2-04144] - CEM-T4 cell lysate. Image from verified customer review.
Immunohistochemistry: SFRS4 Antibody [NBP2-04144] - Samples: FFPE serial sections of human lung carcinoma. Antibody: Affinity purified rabbit anti- SRp75 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: SFRS4 Antibody [NBP2-04144] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRp75 antibody NBP2-04144 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp75 was also immunoprecipitated by rabbit anti-SRp75 antibody NBP2-04143, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
(2 Reviews)
Species: Hu
Applications: WB, IHC, IHC-P, IP
SFRS3 Antibody
(2D2)
H00006428-M08
Western Blot: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of SFRS3 expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/Immunofluorescence: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of monoclonal antibody to SFRS3 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of SFRS3 expression in HeLa (Cat # L013V1).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(2 Reviews)
(22 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
HLA B7 Antibody
NBP2-29931
Western Blot: HLA B7 Antibody [NBP2-29931] - Analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Immunohistochemistry-Paraffin: HLA B7 Antibody [NBP2-29931] - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry: HLA B7 Antibody [NBP2-29931] - Analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Species: Hu
Applications: WB, Flow, IHC-P
hnRNP A1 Antibody
(4B10)
NB100-672
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Whole cell protein from HeLa (lane 1), A431 (lane 2), Hek293 (lane 3) and MCF7 (lane 4) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-hnRNP in 1% milk. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). Both antibodies were detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (4B10) [NB100-672] - The hnRNP A1 (4B10) antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-hnRNP-A1 Clone: 4B10 (NB100-672). Lanes: 1-HeLa, 2-Jurkat, 3-HEK-293T, 4-WiDr, 5-NCI-H1299, 6-F9.
(17 Publications)
Species: Hu, Mu, Bv, Ca
Applications: WB, ELISA, ICC/IF, IP, MiAr
Coagulation Factor
III/Tissue Factor Ant ...
AF2339
Coagulation Factor III/Tissue Factor Antibody
(3 Reviews)
(3 Publications)
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready
hnRNP C1 + C2
Antibody (CL2596)
NBP2-36777
Western Blot: hnRNP C1 + C2 Antibody (CL2596) [NBP2-36777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-HNRNPC monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % inten
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (CL2596) [NBP2-36777] - Staining in U251 cell line showing distinct nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2596) [NBP2-36777] - Staining of human lymph node shows strong nuclear immunoreactivity in lymphocytes.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
Tau Antibody (2E9)
NBP2-25162
Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.
Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.
Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
Bim Antibody
NBP2-53088
Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
S100B Antibody
(15F4NB)
NBP2-45224
Western Blot: S100B Antibody (15F4NB) [NBP2-45224] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) [NBP2-45224] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) [NBP2-45224] - Analysis using Azide Free version of NBP2-45224. S100B (15F4BN) in human brain tissue.
(1 Review)
(1 Publication)
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for SRp55 Antibody (NBP2-04142) (0)

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Reviews for SRp55 Antibody (NBP2-04142) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-04142:
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WB
(2)
ICC
(1)
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Human
(3)
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Images Ratings Applications Species Date Details
Western Blot SRp55 NBP2-04142
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reviewed by:
Anonymous
 WB Human 06/09/2018
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Summary

ApplicationWestern Blot
Sample Tested HeLa cell lysate
SpeciesHuman
LotA1
Immunocytochemistry SRp55 NBP2-04142
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reviewed by:
Anonymous
 ICC Human 11/18/2017
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Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

Comments1X PBS/5% normal serum/0.3% Triton™ X-100
Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
2 hours (25C) secondary (1.5:1000 dilution)
Western Blot SRp55 NBP2-04142
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reviewed by:
Anonymous
 WB Human 01/21/2016
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Summary

ApplicationWestern Blot
Sample TestedHuman lymphocytes
SpeciesHuman
LotA1

Blocking

Blocking Details5% Milk/TBS-T, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:2000, 16H 4C, 5% milk/TBS-T

Secondary Antibody

Secondary DescriptionAmersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)
Secondary Manufacturer Cat#NA934-100UL
Secondary Concentration1:5000

Details

Detection NotesECL, touch exposure

Comments

Comments14ug of wholw cell lysate per lane, 3 different human B-cell lines.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SRp55 Antibody (NBP2-04142) (0)

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Positive Control Lysate(s)

SRp55 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Western Blot SRp55 NBP2-04142
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 Anonymous
06/09/2018
Application: WB
Species: Human
read this review
Immunocytochemistry SRp55 NBP2-04142
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 Anonymous
11/18/2017
Application: ICC
Species: Human
read this review
Western Blot SRp55 NBP2-04142
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 Anonymous
01/21/2016
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol SRSF6
Uniprot