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SRp55 Antibody

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 (3 Reviews)
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Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.
Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: SRp55 Antibody [NBP2-04142] - Staining in human fibroblasts. 1X PBS/5% normal serum/0.3% Triton™ X-100. Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at ...read more
Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified ...read more
Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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SRp55 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to this antibody using the numbering given in entry NP_006266.2 (GeneID 6431).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRSF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 mcg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. SRp55 antibody validated for ICC/IF from a verified customer review.
Control
SRp55 Knockout 293T Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP2-04142 in the following applications:
Publications
Read Publication using
NBP2-04142 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SRp55 Antibody

  • B52
  • FLJ08061
  • pre-mRNA splicing factor SRP55
  • Pre-mRNA-splicing factor SRP55
  • serine/arginine-rich splicing factor 6
  • SFRS6
  • Splicing factor, arginine/serine-rich 6MGC5045
  • splicing factor, arginine/serine-rich, 55 kDa
  • SR splicing factor 6
  • SRP55arginine/serine-rich splicing factor 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: SF2 Antibody [NBP2-20324] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse duodenum. SF2 antibody dilution: 1:500.
(2 Publications)
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Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma-Synuclein antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - Synuclein-gamma Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Synuclein-gamma Antibody [NBP1-31209] - Paraffin-embedded mouse fore brain, gamma Synuclein antibody dilution: 1:500.
Species: Hu, Mu
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Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human Secretin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6387) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Secretin R delta 3/4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western Blot: ARSF Antibody [NBP2-92826] - Analysis of extracts of various cell lines, using ARSF at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: ARSF Antibody [NBP2-92826] - Paraffin-embedded rat lung using ARSF.
Immunohistochemistry-Paraffin: ARSF Antibody [NBP2-92826] - Paraffin-embedded human liver using ARSF.
Species: Hu, Mu, Rt
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Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP2-37888] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human liver.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: RNPS1 Antibody (7G8) [H00010921-M05] - Analysis of RNPS1 expression in rat testis.
Western Blot: RNPS1 Antibody (7G8) [H00010921-M05] - Analysis of RNPS1 expression in HeLa.
Western Blot: RNPS1 Antibody (7G8) [H00010921-M05] - Analysis of RNPS1 expression in PC-12.
(1 Publication)
Species: Hu, Mu, Rt
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Western Blot: SFRS5 Antibody [NBP1-92381] - Analysis in human cell line HeLa.
Immunocytochemistry/Immunofluorescence: SFRS5 Antibody [NBP1-92381] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SFRS5 Antibody [NBP1-92381] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated by SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:100. Lane 2: Antibody dilution 1:250. Lane 3: Antibody dilution 1:500. Lane 4: Antibody dilution 1:1000.
Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - Gadd45a localization in nuclear speckles is RNase sensitive Immunofluorescence confocal microscopy of detergent-extracted RKO cells. Cells were transfected with N-EGFP-Gadd45a and stained with antibodies against SC35and p68. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014500) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing Factor Clone: SC-35 diluted to 1:2,000. The antibody was developed using Goat Anti-Mouse IgG, FITC-conjugate.
(2 Reviews)
(7 Publications)
Species: Hu, Mu, Rt, Am, Dr
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Western Blot: SFRS4 Antibody [NBP2-04144] - J-Lat cells treated with sh vectors targeting SR proteins were induced with Prostratin over 24 hours. Cells were harvested for protein using RIPA. Blots for SRSF4, GFP, HIV p24 GAG protein, and the stain free gel are shown. The J-LAt cell line used had an inducibe HIV construct that when induced would produce GFP as well as HIV proteins such as p24 (GAG). The sh vector was used to knock down the protein. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SFRS4 Antibody [NBP2-04144] - Sections of human lung carcinoma. Antibody: Affinity purified rabbit anti- SRp75 used at a dilution of 1:1000 (1 ug/mL). Detection: DAB. Counterstain: Hematoxylin (blue).
Western Blot: SFRS4 Antibody [NBP2-04144] - CEM-T4 cell lysate. Western blot image submitted by a verified customer review.
(3 Reviews)
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Applications: WB, IHC, IHC-P, IP
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Western Blot: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of SFRS3 expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/Immunofluorescence: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of monoclonal antibody to SFRS3 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: SFRS3 Antibody (2D2) [H00006428-M08] - Analysis of SFRS3 expression in HeLa (Cat # L013V1).
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Species: Hu, Mu, Rt
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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(2G7B10)
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Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
hnRNP A1 Antibody
(4B10)
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Knockdown Validated: hnRNP A1 Antibody (4B10) [NB100-672] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human hnRNP A1 Monoclonal Antibody (Catalog # NB100-672) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for hnRNP A1 at approximately 35 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Whole cell protein from HeLa (lane 1), A431 (lane 2), Hek293 (lane 3) and MCF7 (lane 4) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-hnRNP in 1% milk. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). Both antibodies were detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (4B10) [NB100-672] - The hnRNP A1 (4B10) antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
(21 Publications)
Species: Hu, Mu, Bv, Ca
Applications: WB, ELISA, ICC/IF, IHC, IP, MiAr, KD
Coagulation Factor
III/Tissue Factor [HR ...
DCF300
(1 Review)
(16 Publications)
Species: Hu
hnRNP C1 + C2
Antibody (SN0652)
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Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Tau [p Thr181]
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Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Thr181) Antibody and the same antibody preincubated with blocking peptide.
Immunohistochemistry-Paraffin: Tau [p Thr181] Antibody [NB100-82245] - analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr181) antibody.
Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Western blot analysis of extract from mouse brain tissue using Tau (Ab-181) antibody ( Lane 1 and 2) and Tau (phospho-Thr181) antibody ( Lane 3 and 4).
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, KD
Bim Antibody
NBP1-76963
Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
(7 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
S100B Antibody
(15F9NB)
NBP2-45267
Western Blot: S100B Antibody (15F9NB) [NBP2-45267] - Total protein from human brain (1) and mouse brain (2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in human prostate tissue.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
SRp55 Antibody
NBP2-04142
SRp55 Antibody
Species: Hu, Mu, Bv
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for SRp55 Antibody (NBP2-04142)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Reviews for SRp55 Antibody (NBP2-04142) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-04142:
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WB
(2)
ICC
(1)
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Human
(3)
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Images Ratings Applications Species Date Details
Western Blot SRp55 NBP2-04142
Enlarge 		5
reviewed by:
Anonymous
 WB Human 06/09/2018
View

Summary

ApplicationWestern Blot
Sample Tested HeLa cell lysate
SpeciesHuman
LotA1
Immunocytochemistry SRp55 NBP2-04142
Enlarge 		3
reviewed by:
Anonymous
 ICC Human 11/18/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

Comments1X PBS/5% normal serum/0.3% Triton™ X-100
Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
2 hours (25C) secondary (1.5:1000 dilution)
Western Blot SRp55 NBP2-04142
Enlarge 		5
reviewed by:
Anonymous
 WB Human 01/21/2016
View

Summary

ApplicationWestern Blot
Sample TestedHuman lymphocytes
SpeciesHuman
LotA1

Blocking

Blocking Details5% Milk/TBS-T, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:2000, 16H 4C, 5% milk/TBS-T

Secondary Antibody

Secondary DescriptionAmersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)
Secondary Manufacturer Cat#NA934-100UL
Secondary Concentration1:5000

Details

Detection NotesECL, touch exposure

Comments

Comments14ug of wholw cell lysate per lane, 3 different human B-cell lines.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SRp55 Antibody (NBP2-04142) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

SRp55 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Diseases for SRp55 Antibody (NBP2-04142)

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Blogs on SRp55

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Recent Reviews

4.3
5
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Western Blot SRp55 NBP2-04142
Enlarge
 Anonymous
06/09/2018
Application: WB
Species: Human
read this review
Immunocytochemistry SRp55 NBP2-04142
Enlarge
 Anonymous
11/18/2017
Application: ICC
Species: Human
read this review
Western Blot SRp55 NBP2-04142
Enlarge
 Anonymous
01/21/2016
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol SRSF6
Uniprot