SRp55 Antibody

Images

 
Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.
Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified ...read more
Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SRp55 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to this antibody using the numbering given in entry NP_006266.2 (GeneID 6431).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRSF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
SRp55 Lysate (NBP2-65826)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP2-04142 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SRp55 Antibody

  • B52
  • FLJ08061
  • pre-mRNA splicing factor SRP55
  • Pre-mRNA-splicing factor SRP55
  • serine/arginine-rich splicing factor 6
  • SFRS6
  • Splicing factor, arginine/serine-rich 6MGC5045
  • splicing factor, arginine/serine-rich, 55 kDa
  • SR splicing factor 6
  • SRP55arginine/serine-rich splicing factor 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-04142
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for SRp55 Antibody (NBP2-04142) (0)

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Reviews for SRp55 Antibody (NBP2-04142) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-04142:
Filter by Applications
WB
(2)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot SRp55 NBP2-04142
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reviewed by:
Anonymous
WB Human 06/09/2018
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Summary

ApplicationWestern Blot
Sample Tested HeLa cell lysate
SpeciesHuman
LotA1
Immunocytochemistry SRp55 NBP2-04142
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reviewed by:
Anonymous
ICC Human 11/18/2017
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Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

Comments1X PBS/5% normal serum/0.3% Triton™ X-100
Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
2 hours (25C) secondary (1.5:1000 dilution)
Western Blot SRp55 NBP2-04142
Enlarge
reviewed by:
Anonymous
WB Human 01/21/2016
View

Summary

ApplicationWestern Blot
Sample TestedHuman lymphocytes
SpeciesHuman
LotA1

Blocking

Blocking Details5% Milk/TBS-T, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:2000, 16H 4C, 5% milk/TBS-T

Secondary Antibody

Secondary DescriptionAmersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)
Secondary Manufacturer Cat#NA934-100UL
Secondary Concentration1:5000

Details

Detection NotesECL, touch exposure

Comments

Comments14ug of wholw cell lysate per lane, 3 different human B-cell lines.

Product General Protocols

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Video Protocols

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FAQs for SRp55 Antibody (NBP2-04142) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for SRp55 Antibody (NBP2-04142)

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Diseases for SRp55 Antibody (NBP2-04142)

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Recent Reviews

4.3
5
2
4
0
3
1
2
0
1
0

Anonymous
06/09/2018
Application: WB
Species: Human

Anonymous
11/18/2017
Application: ICC
Species: Human

Anonymous
01/21/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol SRSF6
Uniprot