SC35 Antibody (SR2484)

Western Blot: SC35 Antibody (SR2484) [NBP3-27758] - Western blot analysis of SC35 in C6, MCF-7 lysates

Immunohistochemistry-Paraffin: SC35 Antibody (SR2484) [NBP3-27758] - Immunohistochemistry analysis of paraffin-embedded Human tonsil. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

• Reactivity: Hu, Rt
• Applications: WB, IHC, IP
• Clone: SR2484
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SC35 Antibody (SR2484) Summary

• Description: Novus Biologicals Rabbit SC35 Antibody (SR2484) (NBP3-27758) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human SC35 (Uniprot #: Q01130)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SRSF2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunoprecipitation 1:20
  • Western Blot 1:500-1:1000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for SC35 Antibody (SR2484)

• PR264
• SC35
• SC-35
• serine/arginine-rich splicing factor 2
• SFRS2
• SFRS2A
• splicing component, 35 kDa
• splicing factor SC35
• splicing factor, arginine/serine-rich 2
• SR splicing factor 2
• SRp30b

Background

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRSF2