SATB2 Antibody

Images

 
Western Blot: SATB2 Antibody [NBP1-83619] - Analysis in human cell line HELA.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining in human colon and liver tissues. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human liver shows no positivity as expected.
Simple Western: SATB2 Antibody [NBP1-83619] - Simple Western lane view shows a specific band for SATB2 in 0.1 mg/ml of THP1 (left), HDLM2 (right) lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: SATB2 Antibody [NBP1-83619] - Electropherogram image(s) of corresponding Simple Western lane view. SATB2 antibody was used at 1:50 dilution on THP1 and HDLM2 lysate(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SATB2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAK
Specificity
Specificity of human SATB2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SATB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
SATB2 Lysate (NBP2-65726)
Control Peptide
SATB2 Protein (NBP1-83619PEP)
Publications
Read Publications using NBP1-83619.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 21898641)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SATB2 Antibody

  • DNA-binding protein SATB2
  • FLJ32076
  • KIAA1034FLJ21474
  • MGC119474
  • MGC119477
  • SATB family member 2
  • SATB homeobox 2
  • Special AT-rich sequence-binding protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SATB2 Antibody (NBP1-83619)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SATB2 Antibody (NBP1-83619) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SATB2 Products

Bioinformatics Tool for SATB2 Antibody (NBP1-83619)

Discover related pathways, diseases and genes to SATB2 Antibody (NBP1-83619). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SATB2 Antibody (NBP1-83619)

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Calculators

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Bioinformatics

Gene Symbol SATB2