SATB2 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Analysis in human colon and liver tissues. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human cerebral cortex, colon, liver and rectum using Anti-SATB2 antibody NBP1-83619 (A) shows similar protein ...read more
Western Blot: SATB2 Antibody [NBP1-83619] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human liver shows no positivity as expected.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human colon shows strong nuclear positivity in glandular cells.
Simple Western: SATB2 Antibody [NBP1-83619] - Simple Western lane view shows a specific band for SATB2 in 0.1 mg/ml of THP1 (left), HDLM2 (right) lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: SATB2 Antibody [NBP1-83619] - Electropherogram image(s) of corresponding Simple Western lane view. SATB2 antibody was used at 1:50 dilution on THP1 and HDLM2 lysate(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SATB2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAK
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SATB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Simple Western 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SATB2 Protein (NBP1-83619PEP)
Publications
Read Publications using NBP1-83619.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 21898641)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SATB2 Antibody

  • DNA-binding protein SATB2
  • FLJ32076
  • KIAA1034FLJ21474
  • MGC119474
  • MGC119477
  • SATB family member 2
  • SATB homeobox 2
  • Special AT-rich sequence-binding protein 2

Background

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SATB2 Antibody (NBP1-83619) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SATB2 Products

Array NBP1-83619

Research Areas for SATB2 Antibody (NBP1-83619)

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Blogs on SATB2

There are no specific blogs for SATB2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

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Calculators

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Bioinformatics

Gene Symbol SATB2