SATB1 Antibody

Images

 
Western Blot: SATB1 Antibody [NBP2-15108] - Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody diluted by 1:500
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108] - SATB1 antibody detects SATB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. SATB1 ...read more
Western Blot: SATB1 Antibody [NBP2-15108] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Western Blot: SATB1 Antibody [NBP2-15108] - Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody diluted by 1:500
Western Blot: SATB1 Antibody [NBP2-15108] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108] - Paraffin-embedded TOV-21G xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15108] - SATB1 antibody detects SATB1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SATB1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB, ChIP, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Order Details

SATB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SATB1. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (100%), Bovine (98%), Chicken (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SATB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
ChIP usage reported in scientific literature.
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15108 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SATB1 Antibody

  • DNA-binding protein SATB1
  • SATB homeobox 1
  • SATB1
  • special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
  • Special AT-rich sequence-binding protein 1

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SATB1 Antibody (NBP2-15108) (0)

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Review for SATB1 Antibody (NBP2-15108) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ChIP.

Reviews using NBP2-15108:
Filter by Applications
ChIP
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation SATB1 NBP2-15108
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reviewed by:
Ranveer Singh
ChIP Human 06/18/2014
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCross-linked chromatin from HEK 293T cells
SpeciesHuman

Primary Anitbody

Dilution Ratio2ug for 400 ul lysate

Details

Detection Notesreal time quantitative PCR (SYBR green)
Fixation/Cross-LinkingReverse cross linking was done in elution buffer with NaCl at 65 degree O/N
DNS SharingDiagenode Bioruptor was used in "High" setting, for 7 mins X 3 time to get approx 500bp fragments
Immunoprecipitation DetailsMagnetic Dynabeads (Protein G)
Crosslinking/Reversal Details1% formaldehyde for 10 mins at room temperature
DNA Prurification DetailsDNA was purified using PCR Purification Kit (Qiagen)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SATB1 Antibody (NBP2-15108) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SATB1 Antibody (NBP2-15108)

Discover related pathways, diseases and genes to SATB1 Antibody (NBP2-15108). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SATB1 Antibody (NBP2-15108)

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Pathways for SATB1 Antibody (NBP2-15108)

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Recent Reviews

5
5
1
4
0
3
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2
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1
0

Ranveer Singh
06/18/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol SATB1
Entrez
Uniprot