SATB1 Antibody

Western Blot: SATB1 Antibody [NBP2-15107] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107] - Mouse lymph node. SATB1 stained by SATB1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate

Chromatin Immunoprecipitation: SATB1 Antibody [NBP2-15107] - Cross-linked ChIP was performed with Jurkat chromatin extract and 5 ug of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.ChIP experiment and primer designs are based on Nucleic Acids Res. 2011 Jun;39(11):4640-52.

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Immunohistochemistry-Paraffin: SATB1 Antibody [NBP2-15107] - Mouse thymus gland. SATB1 stained by SATB1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citra

Immunoprecipitation: SATB1 Antibody [NBP2-15107] - 293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 ug of SATB1 antibody 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Western Blot: SATB1 Antibody [NBP2-15107] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (NBP2-15107) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: SATB1 Antibody [NBP2-15107] - SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SATB1 stained by SATB1 antibody [C2C3], C-term (NBP2-15107) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Ch, RM
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SATB1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SATB1. The exact sequence is proprietary.
• Predicted Species: Rat (96%), Chicken (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SATB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
• Application Notes: ChIP assay-Assay dependent
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SATB1 Antibody

• DNA-binding protein SATB1
• SATB homeobox 1
• SATB1
• special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's)
• Special AT-rich sequence-binding protein 1

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SATB1
• Uniprot:
  • Q01826()