SASH1 Antibody

Images

 
Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate from HeLa cells. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody NBP1-26651.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

SASH1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human SAM and SH3 domain containing 1 (NP_056093.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SASH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 3-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-26650 in the following applications:

Publications
Read Publications using
NBP1-26650 in the following applications:

  • WB
    2 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, Crab-eating acaque, African elephant and Northern white-cheeked gibbon. Mouse reactivity reported in scientific literature (PMID: 23235829).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SASH1 Antibody

  • dJ323M4
  • dJ323M4.1
  • KIAA0790RP3-323M4.1
  • PEPE1
  • Proline-glutamate repeat-containing protein
  • SAM and SH3 domain containing 1,2500002E12Rik
  • SAM and SH3 domain-containing protein 1
  • SH3D6A

Background

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SASH1 Antibody (NBP1-26650)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Human
(1)
Mouse
(1)
All Species

Review for SASH1 Antibody (NBP1-26650) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-26650:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 04/29/2010
View

Summary

ApplicationWestern Blot
Sample TestedHaCat (a keratinocyte cell line) whole cell lysate
SpeciesHuman
CommentsThis SASH1 antibody worked very well in a Western blot. We also tested it in Immunofluorescence and IHC applications but had minimal success.

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Comments

CommentsThis SASH1 antibody worked very well in a Western blot. We also tested it in Immunofluorescence and IHC applications but had minimal success.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SASH1 Antibody (NBP1-26650) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SASH1 Antibody (NBP1-26650)

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Research Areas for SASH1 Antibody (NBP1-26650)

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Blogs on SASH1

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
04/29/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol SASH1
Entrez
Uniprot