SASH1 Antibody

Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: SASH1 Antibody [NBP1-26650] - Whole cell lysate from HeLa cells. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody NBP1-26651.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SASH1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human SAM and SH3 domain containing 1 (NP_056093.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SASH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in IHC reported in scientific literature (PMID: 31067462).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23235829).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SASH1 Antibody

• dJ323M4
• dJ323M4.1
• KIAA0790RP3-323M4.1
• PEPE1
• Proline-glutamate repeat-containing protein
• SAM and SH3 domain containing 1,2500002E12Rik
• SAM and SH3 domain-containing protein 1
• SH3D6A

Background

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SASH1 Antibody (NBP1-26650)(6)

Publications using NBP1-26650

• Xu Z, Li Y, Wang D et al. Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock in mouse model Int. J. Mol. Med. 2020-06-19 [PMID: 32582980] (IHC, WB, Mouse)
• Franke Fc, Slusarenko Bo, Engleitner T et Al. Novel role for CRK adaptor proteins as essential components of SRC/FAK signaling for epithelial-mesenchymal transition and colorectal cancer aggressiveness Int. J. Cancer 2020-03-09 [PMID: 32147820] (IB, Human)
• Kretschmar C, Pena-Oyarzun D, Hernando C et al. Endothelial Sash1 Is Required for Lung Maturation through Nitric Oxide Signaling Cell Rep 2019-05-07 [PMID: 31067462] (IHC, WB, Human, Mouse)
• Weidmann H, Touat-Hamici Z, Durand H et al. SASH1, a new potential link between smoking and atherosclerosis. Atherosclerosis. [PMID: 26318107] (WB, Human)
• Ascano M, Mukherjee N, Bandaru P et al. FMRP targets distinct mRNA sequence elements to regulate protein expression Nature 2012-12-12 [PMID: 23235829] (WB, Mouse)
• Coulombe P Endothelial regulation in cell fate specification during embryonic development of the lung and the hematopoietic system Thesis 2021-01-01

Review for SASH1 Antibody (NBP1-26650) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: anne brauweiler WB Human 04/29/2010

Summary

• Application: Western Blot
• Sample Tested: HaCat (a keratinocyte cell line) whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: SASH1
• Entrez:
  • 23328(Human)
• Uniprot:
  • O94885(Human)