SAE2 Antibody

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Western Blot: SAE2 Antibody [NB120-22104] - Western bot analysis of SAE2 in HeLa cell lysate using NB120-22104 at 1.0 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SAE2 Antibody Summary

Immunogen
A synthetic peptide within amino acid residues 160-210 of human SAE2 was used as immunogen.
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBA2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB120-22104 in the following applications:

  • WB
    4 publications

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for SAE2 Antibody

  • Anthracycline-associated resistance ARX
  • ARX
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ13058
  • HRIHFB2115
  • SAE2SUMO-1 activating enzyme subunit 2
  • SUMO1 activating enzyme subunit 2
  • SUMO-activating enzyme subunit 2
  • UBA2, ubiquitin-activating enzyme E1 homolog
  • Ubiquitin-like 1-activating enzyme E1B
  • ubiquitin-like modifier activating enzyme 2
  • UBLE1B

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAE2 Antibody (NB120-22104)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBA2
Entrez
Uniprot