SAE2 Antibody

Western Blot: SAE2 Antibody [NB120-22104] - Western bot analysis of SAE2 in HeLa cell lysate using NB120-22104 at 1.0 ug/ml.

Product Discontinued

SAE2 Antibody Summary

• Immunogen: A synthetic peptide within amino acid residues 160-210 of human SAE2 was used as immunogen.
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 2 ug/ml
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for SAE2 Antibody

• Anthracycline-associated resistance ARX
• ARX
• EC 6.3.2
• EC 6.3.2.-
• FLJ13058
• HRIHFB2115
• SAE2SUMO-1 activating enzyme subunit 2
• SUMO1 activating enzyme subunit 2
• SUMO-activating enzyme subunit 2
• UBA2, ubiquitin-activating enzyme E1 homolog
• Ubiquitin-like 1-activating enzyme E1B
• ubiquitin-like modifier activating enzyme 2
• UBLE1B

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAE2 Antibody (NB120-22104)(4)

Publications using NB120-22104

• Sidik SM, Salsman J, Dellaire G, Rohde JR. Shigella Infection Interferes with SUMOylation and Increases PML-NB Number PLoS ONE 2015-04-08 [PMID: 25848798] (WB, Human)
  
  Details:
  SAE2 antibody was used for WB on whole cell lysates from HeLa cells which were infected for 1, 2 or 3 hours with M90T, BS176 and UI strains of Shigella bearing a plasmid expressing the afaE adhesin (Fig 4).
• Pal S, Santos A, Rosas JM et al. Influenza A virus interacts extensively with the cellular SUMOylation system during infection. Virus Res. 2011-06-01 [PMID: 21376763] (WB, Human)
• La Salle S, Sun F, Zhang XD et al. Developmental control of sumoylation pathway proteins in mouse male germ cells. Dev Biol. 2008-09-01 [PMID: 18602382] (WB)
  
  Details:
  SAE2 (IMG-5111A), PIASg (IMG-290), SENP1 CT (IMG-521). WB: Male germ cells; leptotene/zygotene, prepubertal pachytene, and adult pachytene spermatocytes, Fig 5.
• Rosendorff A, Sakakibara S, Lu S et al. NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression. Proc Natl Acad Sci U S A. 2006-04-04 [PMID: 16567619] (WB)
  
  Details:
  WB (293T cells transfected with NXP2): Fig 4A, Table 2 (band detected at 95 kD).

Bioinformatics

• Gene Symbol: UBA2
• Entrez:
  • 50995(Mouse)
  • 308508(Rat)
• Uniprot:
  • Q9UBT2(Human)