Recombinant Human UBE2I/Ubc9 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human UBE2I/Ubc9 Protein, CF Summary

Details of Functionality
Recombinant Human UBE2I/Ubc9 is a member of the SUMO-conjugating (E2) enzyme family that receives SUMO from a SUMO-activating (E1) enzyme and subsequently conjugates SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2I/Ubc9 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2I
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E2-645 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UBE2I/Ubc9 Protein, CF

  • C358B7.1
  • EC 6.3.2
  • EC 6.3.2.-
  • p18
  • SUMO-1-protein ligase
  • SUMO-protein Ligase
  • Ubc9
  • UBC9SUMO-conjugating enzyme UBC9
  • UBCE9
  • UBE2I
  • Ubiquitin carrier protein 9
  • Ubiquitin carrier protein I
  • ubiquitin conjugating enzyme 9
  • Ubiquitin-conjugating enzyme E2 I
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme UbcE2A
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • ubiquitin-protein ligase E2I
  • Ubiquitin-protein ligase I

Background

Ubiquitin-conjugating Enzyme E2I (UBE2I), also known as Ubiquitin-conjugating Enzyme 9 (Ubc9), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. Human UBE2I/Ubc9 shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2I/Ubc9 catalyzes the addition of the Ubiquitin-like protein SUMO to target proteins (1,2). SUMO is transferred from a Ubiquitin-like activating (E1) enzyme heterodimer consisting of SAE1 and UBA2 to Cys93 of UBE2I/Ubc9 (3). In contrast to most Ubiquitin-conjugating (E2) enzymes that function in a complex with Ubiquitin ligases (E3s), UBE2I/Ubc9 can interact directly with protein substrates and may also play a role in substrate recognition (4). UBE2I/Ubc9 mediates the SUMOylation of a variety of proteins including RanGAP1, HDAC4, and PML (5,6). Post-translational modifications of UBE2I/Ubc9, such as auto-SUMOylation at Lys14 or Ser71 phosphorylation by CDC2/CDK1, alter UBE2I/Ubc9 catalytic activity and target protein recognition (5,7). UBE2I/Ubc9-dependent SUMOylation reduces the levels of the stem cell marker Nanog, implicating it in embryonic stem cell pluripotency maintenance (8). SUMOylation also regulates the protein levels of tumor suppressors and oncogenes, and UBE2I/Ubc9 dysregulation is thought to contribute to the pathogenesis of human cancers (9).

  1. Desterro, J.M. et al. (1999) J. Biol. Chem. 274:10618.
  2. Johnson, E.S. & G. Blobel (1997) J. Biol. Chem. 272:26799.
  3. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  4. Yunus, A.A. & C.D. Lima (2006) Nat. Struct. Mol. Biol. 13:491.
  5. Knipscheer, P. et al. (2008) Mol. Cell 31:371.
  6. Duprez, E. et al. (1999) J. Cell Sci. 112:381.
  7. Su, Y.F. et al. (2012) PLoS One 7:e34250.
  8. Wu, Y. et al. (2012) PLoS One 7:e39606.
  9. Mo, Y.Y. et al. (2005) Oncogene 24:2677.
Additional References
  1. Joana M.P, et al. (1997) FEBS. Lett. 417:3168-3179
  2. Ito K., et al. (1999) Cytogenet. Cell. 84:99-104
  3. Liu Q., et al. (1999) J. Biol. Chem. 274:16979-16987
  4. Matushewski K., et al. (1996) J. Biol. Chem. 271:2789-2794
  5. Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
  6. Tatham M.H., et al. (2003) Biochem. 42:3168-3179
  7. Tong H., et al. (1997) J. Biol. Chem. 272:21381-21387
  8. Watanabe T.K., et al. (1996) Cytogen. Cell. Genet. 72:86-89
  9. Yasugi T and Howley P.M (1996) Nuc. Acid. Res. 24:2005-2010
 

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E2-645
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Publications for UBE2I/Ubc9 (E2-645)(5)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 1 application: Bioassay.


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Bioassay
(5)
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(3)
N/A
(1)
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Bioinformatics

Gene Symbol UBE2I
Entrez
Uniprot