Recombinant Human UBE2I/Ubc9 Protein, CF


There are currently no images for UBE2I/Ubc9 (E2-645).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human UBE2I/Ubc9 Protein, CF Summary

Details of Functionality
Recombinant Human UBE2I/Ubc9 is a member of the SUMO-conjugating (E2) enzyme family that receives SUMO from a SUMO-activating (E1) enzyme and subsequently conjugates SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 μM.
E. coli-derived human UBE2I/Ubc9 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain


Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
E2-645 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UBE2I/Ubc9 Protein, CF

  • C358B7.1
  • EC 6.3.2
  • EC 6.3.2.-
  • p18
  • SUMO-1-protein ligase
  • SUMO-protein Ligase
  • Ubc9
  • UBC9SUMO-conjugating enzyme UBC9
  • UBCE9
  • UBE2I
  • Ubiquitin carrier protein 9
  • Ubiquitin carrier protein I
  • ubiquitin conjugating enzyme 9
  • Ubiquitin-conjugating enzyme E2 I
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme UbcE2A
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • ubiquitin-protein ligase E2I
  • Ubiquitin-protein ligase I


Ubiquitin-conjugating Enzyme E2I (UBE2I), also known as Ubiquitin-conjugating Enzyme 9 (Ubc9), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. Human UBE2I/Ubc9 shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2I/Ubc9 catalyzes the addition of the Ubiquitin-like protein SUMO to target proteins (1,2). SUMO is transferred from a Ubiquitin-like activating (E1) enzyme heterodimer consisting of SAE1 and UBA2 to Cys93 of UBE2I/Ubc9 (3). In contrast to most Ubiquitin-conjugating (E2) enzymes that function in a complex with Ubiquitin ligases (E3s), UBE2I/Ubc9 can interact directly with protein substrates and may also play a role in substrate recognition (4). UBE2I/Ubc9 mediates the SUMOylation of a variety of proteins including RanGAP1, HDAC4, and PML (5,6). Post-translational modifications of UBE2I/Ubc9, such as auto-SUMOylation at Lys14 or Ser71 phosphorylation by CDC2/CDK1, alter UBE2I/Ubc9 catalytic activity and target protein recognition (5,7). UBE2I/Ubc9-dependent SUMOylation reduces the levels of the stem cell marker Nanog, implicating it in embryonic stem cell pluripotency maintenance (8). SUMOylation also regulates the protein levels of tumor suppressors and oncogenes, and UBE2I/Ubc9 dysregulation is thought to contribute to the pathogenesis of human cancers (9).

  1. Desterro, J.M. et al. (1999) J. Biol. Chem. 274:10618.
  2. Johnson, E.S. & G. Blobel (1997) J. Biol. Chem. 272:26799.
  3. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  4. Yunus, A.A. & C.D. Lima (2006) Nat. Struct. Mol. Biol. 13:491.
  5. Knipscheer, P. et al. (2008) Mol. Cell 31:371.
  6. Duprez, E. et al. (1999) J. Cell Sci. 112:381.
  7. Su, Y.F. et al. (2012) PLoS One 7:e34250.
  8. Wu, Y. et al. (2012) PLoS One 7:e39606.
  9. Mo, Y.Y. et al. (2005) Oncogene 24:2677.
Additional References
  1. Joana M.P, et al. (1997) FEBS. Lett. 417:3168-3179
  2. Ito K., et al. (1999) Cytogenet. Cell. 84:99-104
  3. Liu Q., et al. (1999) J. Biol. Chem. 274:16979-16987
  4. Matushewski K., et al. (1996) J. Biol. Chem. 271:2789-2794
  5. Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
  6. Tatham M.H., et al. (2003) Biochem. 42:3168-3179
  7. Tong H., et al. (1997) J. Biol. Chem. 272:21381-21387
  8. Watanabe T.K., et al. (1996) Cytogen. Cell. Genet. 72:86-89
  9. Yasugi T and Howley P.M (1996) Nuc. Acid. Res. 24:2005-2010

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, IF
Species: Hu
Applications: WB, ICC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu, Rt
Applications: WB, ELISA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ELISA, IP, RNAi
Species: Hu
Applications: Enzyme Activity

Publications for UBE2I/Ubc9 (E2-645)(5)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 1 application: Bioassay.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for UBE2I/Ubc9 (E2-645) (0)

There are no reviews for UBE2I/Ubc9 (E2-645). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for UBE2I/Ubc9 (E2-645) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for UBE2I/Ubc9 (E2-645)

Discover related pathways, diseases and genes to UBE2I/Ubc9 (E2-645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UBE2I/Ubc9 (E2-645)

Discover more about diseases related to UBE2I/Ubc9 (E2-645).

Pathways for UBE2I/Ubc9 (E2-645)

View related products by pathway.

PTMs for UBE2I/Ubc9 (E2-645)

Learn more about PTMs related to UBE2I/Ubc9 (E2-645).

Blogs on UBE2I/Ubc9

There are no specific blogs for UBE2I/Ubc9, but you can read our latest blog posts.

Customers Who Bought This Also Bought

p19ARF Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human UBE2I/Ubc9 Protein, CF and receive a gift card or discount.


Gene Symbol UBE2I