SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) - Azide and BSA Free

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - SAE1 monoclonal antibody (M01), clone 1G4-1G5 Analysis of SAE1 expression in HeLa.

Immunocytochemistry/Immunofluorescence: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Analysis of monoclonal antibody to SAE1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Analysis of monoclonal antibody to SAE1 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.

Sandwich ELISA: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Detection limit for recombinant GST tagged SAE1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1G4-1G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) - Azide and BSA Free Summary

• Immunogen: SAE1 (AAH00344, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
• Specificity: SAE1 - SUMO-1 activating enzyme subunit 1
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) - Azide and BSA Free

• AOS1
• AOS1activator of SUMO1
• FLJ3091
• HSPC140
• SAE1
• SUA1
• SUA1sentrin/SUMO-activating protein AOS1
• SUMO Activating Enzyme E1 (SAE1/UBA2)
• SUMO-1 activating enzyme E1 N subunit
• SUMO1 activating enzyme subunit 1
• SUMO-1 activating enzyme subunit 1
• SUMO-activating enzyme subunit 1
• UBA2
• Ubiquitin-like 1-activating enzyme E1A
• ubiquitin-like protein SUMO-1 activating enzyme
• UBLE1A

Background

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUMO Activating Enzyme E1 (SAE1) Antibody (H00010055-M01)(2)

Publications using H00010055-M01

• Ge Y, Lu X, Shu X et al. Clinical characteristics of anti-SAE antibodies in Chinese patients with dermatomyositis in comparison with different patient cohorts. Sci Rep 2017-03-15 [PMID: 28298642]
• Fujimoto M, Matsushita T, Hamaguchi Y et al. Autoantibodies to small ubiquitin-like modifier activating enzymes in Japanese patients with dermatomyositis: comparison with a UK Caucasian cohort. Ann Rheum Dis. 2012-07-26 [PMID: 22843487]

Bioinformatics

• Gene Symbol: SAE1
• Entrez:
  • 10055(Human)
• Uniprot:
  • AAH00344(Human)