S100P Antibody (S100P/7978R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human bladder stained with S100P antibody (S100P/7978R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human placenta stained with S100P antibody (S100P/7978R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: S100P/7978R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

S100P Antibody (S100P/7978R) - Azide and BSA Free Summary

• Description: Positive Controls: Human pancreatic adenocarcinoma, placenta or spleen tissue.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-95) of human S100P protein (exact sequence is proprietary)
• Localization: Nucleus. Cytoplasm. Colocalizes with S100PBP in the nucleus.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: S100P
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for S100P Antibody (S100P/7978R) - Azide and BSA Free

• MIG9
• migration-inducing gene 9
• Protein S100-E
• S100 calcium binding protein P
• S100 calcium-binding protein Pprotein S100-P
• S100E
• S100P

Background

The protein encoded by the S100P gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100P