S100P Antibody (357517) [Unconjugated]

Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100P was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF, KO
• Clone: 357517
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

S100P Antibody (357517) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human S100P
  Met1-Lys95
  Accession # P25815
• Specificity: Detects human S100P in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) S100A10 or rhS100B is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100P
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100P Antibody (357517) [Unconjugated]

• MIG9
• migration-inducing gene 9
• Protein S100-E
• S100 calcium binding protein P
• S100 calcium-binding protein Pprotein S100-P
• S100E
• S100P

Background

Human S100P is a 22 kDa, homodimeric member of the S100 family of calcium-binding proteins. The S100 family currently has more than 20 members and belongs to the EF-hand superfamily of molecules. S100P is 95 aa in length and contains short, sequential modules. There is an N-terminal alpha -helix, a unique EF-hand motif, an alpha -helix, a linker region, an alpha -helix, a classic EF-hand motif and a C-terminal alpha -helix. The EF-hand motif binds calcium, which likely alters molecular conformation. The rearranged S100P now binds ligand with the linker region. Human S100P shares 47% aa sequence identity with rat S100P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100P Antibody (MAB2957)(7)

Publications using MAB2957

• Dan Zhang, Mi Han, Mingjuan Zhou, Mengyu Liu, Yan Li, Bufang Xu et al. Down-regulation of S100P induces apoptosis in endometrial epithelial cell during GnRH antagonist protocol Reproductive Biology and Endocrinology 2021-12-01 [PMID: 34215278]
• M Akita, R Sawada, M Komatsu, N Suleman, T Itoh, T Ajiki, N Heaton, T Fukumoto, Y Zen An immunostaining panel of C-reactive protein, N-cadherin, and S100P is useful for intrahepatic cholangiocarcinoma subtyping Human pathology, 2020-12-13;0(0):. 2020-12-13 [PMID: 33321161] (IHC, Human)
• M Akita, K Fujikura, T Ajiki, T Fukumoto, K Otani, T Azuma, T Itoh, Y Ku, Y Zen Dichotomy in intrahepatic cholangiocarcinomas based on histologic similarities to hilar cholangiocarcinomas Mod. Pathol, 2017-03-24;0(0):. 2017-03-24 [PMID: 28338651] (IHC, Human)
• M Chiba, H Imazu, M Kato, K Ikeda, H Arakawa, T Kato, K Sumiyama, S Homma Novel quantitative analysis of the S100P protein combined with endoscopic ultrasound-guided fine needle aspiration cytology in the diagnosis of pancreatic adenocarcinoma Oncol. Rep, 2017-02-21;0(0):. 2017-02-21 [PMID: 28260012] (ELISA Development (Detection), Human)
• Zhang , Dan, Ma , Chengbin, Sun , Xiaoli, Xia , Hexia, Zhang , Wei S100P expression in response to sex steroids during the implantation window in human endometrium. Reprod Biol Endocrinol, 2012-12-07;10(0):106. 2012-12-07 [PMID: 23216986] (IHC, In-Cell Western (ICW), Human)
• S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis. Clin. Cancer Res., 2012-06-20;18(16):4356-64. 2012-06-20 [PMID: 22718861] (ELISA Development, IHC-P, Western Blot, Human)
• Feng W, Brown RE, Trung CD Morphoproteomic profile of mTOR, Ras/Raf kinase/ERK, and NF-kappaB pathways in human gastric adenocarcinoma. Ann. Clin. Lab. Sci., 2008-01-01;38(3):195-209. 2008-01-01 [PMID: 18715846] (IHC-P, Human)

Bioinformatics

• Gene Symbol: S100P
• Uniprot:
  • P25815()