S100P Antibody (357517) [Unconjugated]

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S100P was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Mouse Anti-Human S100P Monoclonal Antibody (Catalog # MAB2957) for 3 hours at room temperature. Cells were ...read more
Genetic Strategies: Western blot shows lysates of HT‑29 human colon adenocarcinoma parental cell line and S100P knockout HT‑29 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
S100P was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT‑29 Human Cell Line cell line using Mouse Anti-Human S100P Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, KO
Clone
357517
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

S100P Antibody (357517) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human S100P
Met1-Lys95
Accession # P25815
Specificity
Detects human S100P in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) S100A10 or rhS100B is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
S100P
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
Positive Control
S100P Lysate (NBP2-65457)
Publications
Read Publications using
MAB2957 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100P Antibody (357517) [Unconjugated]

  • MIG9
  • migration-inducing gene 9
  • Protein S100-E
  • S100 calcium binding protein P
  • S100 calcium-binding protein Pprotein S100-P
  • S100E
  • S100P

Background

Human S100P is a 22 kDa, homodimeric member of the S100 family of calcium-binding proteins. The S100 family currently has more than 20 members and belongs to the EF-hand superfamily of molecules. S100P is 95 aa in length and contains short, sequential modules. There is an N-terminal alpha -helix, a unique EF-hand motif, an alpha -helix, a linker region, an alpha -helix, a classic EF-hand motif and a C-terminal alpha -helix. The EF-hand motif binds calcium, which likely alters molecular conformation. The rearranged S100P now binds ligand with the linker region. Human S100P shares 47% aa sequence identity with rat S100P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100P Antibody (MAB2957)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA detection, IHC, IHC Paraffin-embedded, WB, in-cell western.


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ELISA detection
(1)
IHC
(1)
IHC Paraffin-embedded
(1)
WB
(1)
in-cell western
(1)
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Human
(5)
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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol S100P
Entrez
Uniprot