S100A8/A9 Antibody (27E10)

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Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
27E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

S100A8/A9 Antibody (27E10) Summary

Immunogen
Human blood monocytes
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
S100A8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
W: non-reduced;~22 kDa; doesn't recognize the single proteins S100A8 and S100A9. For IHC-F: acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon).
Publications
Read Publications using
NBP1-60157 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for S100A8/A9 Antibody (27E10)

  • 60B8AG
  • CAGA
  • CFAG
  • CGLA
  • CP-10
  • L1Ag
  • MA387
  • MIF
  • MRP8
  • NIF
  • P8
  • S100 calcium binding protein A8
  • S100A8

Background

The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A8/A9 Antibody (NBP1-60157)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-60157 Applications Species
Molin S, Merl J, Dietrich KA et al. The hand eczema proteome: imbalance of epidermal barrier proteins. Br. J. Dermatol. 2014 Sep 21 [PMID: 25244099] (IHC-P, Human)

Details:
S100A8/A9 antibody used for IHC on formalin-fixed paraffin-embedded skin biopsy samples from human chronic hand eczema (CHE) patients. Antibody used at 1:10 dilution and detected via LSAB method with DAKO REAL? Detection System, Alkaline Phosphatase/RED, Rabbit/Mouse (Figure 2).
IHC-P Human
Zhang H, Maric I, Diprima MJ et al. Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer. Blood. 2013 Aug 15 [PMID: 23757729]
Zhu L, Okano S, Takahara M et al. Expression of S100 Protein Family Members in Normal Skin and Sweat Gland Tumors. J Dermatol Sci 2013 Mar 16 [PMID: 23623205] (IHC-P, Human) IHC-P Human
Champaiboon C, Sappington KJ, Guenther BD, Ross KF, Herzberg MC. Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion. J Biol Chem;284(11):7078-90. 2009 Mar 13. [PMID: 19122197]
Kuhn A, Sonntag M, Sunderkotter C, Lehmann P, Vestweber D, Ruzicka T. Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus. Br J Dermatol;146(5):801-9. 2002 May. [PMID: 12000376]
Hessian PA, Fisher L. The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9) Antibody recognition, epitope definition and the implications for structure. Eur J Biochem;268(2):353-63. 2001 Jan. [PMID: 11168370]
Zwadlo G, Schlegel R, Sorg C. A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol;137(2):512-8. 1986 Jul 15. [PMID: 3722815]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol S100A8