S100A8/S100A9 Heterodimer Antibodies

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue fibroblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue fibroblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="S100A8/S100A9 Heterodimer was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45702) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue fibroblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB002) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="S100A8/S100A9 Heterodimer was detected in immersion fixed HL60 human promyelocytic leukemia cell line using Rabbit Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45701) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood monocytes (PBMC) were stimulated with LPS (1 μg/mL) and monensin overnight, then stained with either (A) Mouse Anti-Human S100A8/A9 Monoclonal Antibody (Catalog # MAB45703) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />