S100A7/Psoriasin Antibody (577513) [Unconjugated]

S100A7 was detected in immersion fixed paraffin-embedded sections of human skin (hair follicle) using Mouse Anti-Human S100A7 Monoclonal Antibody (Catalog # MAB4475) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 577513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

S100A7/Psoriasin Antibody (577513) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human S100A7
  Met1-Gln101
  Accession # P31151
• Specificity: Detects human S100A7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A8, A9, A10, A11, A14, A16, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A7/Psoriasin Antibody (577513) [Unconjugated]

• HID 5
• HID5
• HID-5
• protein S100-A7
• PSOR1
• psoriasin 1
• Psoriasin
• S100 calcium binding protein A7 (psoriasin 1)
• S100 calcium binding protein A7
• S100 calcium-binding protein A7 (psoriasin 1)
• S100 calcium-binding protein A7
• S100A7
• S100A7c

Background

S100A7 (also Psoriasin) is an 11-13 kDa member of the S101 family, EF-hand superfamily of Ca-binding proteins. It is produced by keratinocytes and exists as both an intracellular and extracellular polypeptide. Intercellularly, it binds to E/FABP and may participate in fatty acid metabolism. Extracellularly, it is both monomeric and heterooligomeric and exhibits antibacterial properties. Human S100A7 is 101 amino acids (aa) in length. It contains two EF-hand motifs (aa 13-48 and 50-85), three zinc-binding His residues, one high-affinity Ca-binding site (aa 63-74) and an overlapping antibacterial sequence (aa 35-80). There appear to be no meaningful rodent structural orthologs. Human S100A7 is 93% aa identical to human S100A15/7A, and the S100A7 gene has a duplication (S100A7B) that is 54% aa identical to S100A7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A7/Psoriasin Antibody (MAB4475)(1)

Publication using MAB4475

• J Chorachoo, S Lambert, T Furnholm, L Roberts, L Reingold, S Auepemkiat, SP Voravuthik, A Johnston The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis PLoS ONE, 2018-10-15;13(10):e0205340. 2018-10-15 [PMID: 30321197] (IHC, Human)

Bioinformatics

• Gene Symbol: S100A7
• Uniprot:
  • P31151()