S100A2 Antibody - BSA Free

Western Blot: S100 alpha 2 Antibody [NBP2-20247] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: S100A2 Antibody [NBP2-20247] - A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: S100A2 protein stained by S100A2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: S100A2 Antibody [NBP2-20247] - Human normal kidney. S100A2 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

S100A2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit S100A2 Antibody - BSA Free (NBP2-20247) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human S100A2. The exact sequence is proprietary.
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: S100A2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for S100A2 Antibody - BSA Free

• CAN19
• CAN19protein S100-A2
• MGC111539
• S100 calcium binding protein A2
• S100 calcium-binding protein A2Protein S-100L
• S100A2
• S100L

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: S100A2
• Uniprot:
  • P29034()