Recombinant Mouse S100A1 His Protein

SDS-Page: Recombinant Mouse S100A1 His Protein [NBP1-72335] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Mu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Mouse S100A1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-94 of Mouse S100A1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSELESAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSGFLDVQK DADAVDKVMK ELDENGDGEV DFKEYVVLVA ALTVACNNFF WETS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: S100A1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 12.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mouse S100A1 His Protein

• protein S100-A1
• S100 alpha
• S100 calcium binding protein A1
• S100 calcium-binding protein A1S100
• S-100 protein alpha chain
• S-100 protein subunit alpha
• S100 protein, alpha polypeptide
• S100A
• S100A1
• S100-alpha

Background

S100 calcium binding protein A1, also known as S100A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A1 is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Recombinant mouse S100A1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: S100A1