RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

Western Blot: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Human lymphoma tissue using anti-RTN4IP1 mouse monoclonal antibody.

Flow Cytometry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RTN4IP1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RTN4IP1 mouse monoclonal antibody.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RTN4IP1 mouse monoclonal antibody.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RTN4IP1 mouse monoclonal antibody.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Human Kidney tissue using anti-RTN4IP1 mouse monoclonal antibody.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Human lymph node tissue using anti-RTN4IP1 mouse monoclonal antibody.

Immunohistochemistry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Staining of paraffin-embedded Human pancreas tissue using anti-RTN4IP1 mouse monoclonal antibody.

Flow Cytometry: RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free [NBP2-73952] - Analysis of Jurkat cells, using anti-RTN4IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human RTN4IP1 (NP_116119) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RTN4IP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 38.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RTN4IP1 Antibody (OTI3B2) - Azide and BSA Free

• MGC12934
• NIMPNOGO-interacting mitochondrial protein
• reticulon 4 interacting protein 1
• reticulon-4-interacting protein 1, mitochondrial

Background

RTN4IP1 encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RTN4IP1