p53R2 Antibody

Western blot shows lysates of HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human p53R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

p53R2 Antibody Summary

• Immunogen: E. coli-derived recombinant human p53R2
  Met1-Lys180
  Accession # Q7LG56
• Specificity: Detects human p53R2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RRM2B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p53R2 Antibody

• EC 1.17.4.1
• MGC102856
• MGC42116
• MTDPS8A
• MTDPS8B
• p53-inducible ribonucleotide reductase small subunit 2 homolog
• p53-inducible ribonucleotide reductase small subunit 2-like protein
• p53R2
• P53R2DKFZp686M05248
• PEOA5
• ribonucleoside-diphosphate reductase subunit M2 B
• ribonucleotide reductase M2 B (TP53 inducible)
• RRM2B
• TP53-inducible ribonucleotide reductase M2 B

Background

The p53-inducible ribonucleotide reductase (p53R2) shares similarity with the R2 subunit of ribonucleotide reductase. Both function to provide dNTPs for DNA synthesis. p53R2 is induced in response to genotoxic stress, whereas the R2 functions under homeostatic conditions. When the expression of p53R2 is decreased, cells exhibit decreased ribonucleotide reductase activity, DNA repair activity, and cellular survival in response to genotoxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p53R2 Antibody (AF3788)(1)

Publication using AF3788

• FI Thege, II Cardle, CN Gruber, MJ Siemann, S Cong, K Wittmann, J Love, BJ Kirby Acquired chemoresistance drives spatial heterogeneity, chemoprotection and collective migration in pancreatic tumor spheroids PLoS ONE, 2022-05-26;17(5):e0267882. 2022-05-26 [PMID: 35617275] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RRM2B
• Uniprot:
  • Q7LG56()