RPSA Antibody (JF0955)

Western Blot: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: A431

Immunocytochemistry/Immunofluorescence: RPSA Antibody (JF0955) [NBP2-67117] - Staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.

Flow Cytometry: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of MCF-7 cells with RPSA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: RPSA Antibody (JF0955) [NBP2-67117] - Staining RPSA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RPSA Antibody (JF0955) [NBP2-67117] - Staining RPSA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RPSA Antibody (JF0955) [NBP2-67117] - Staining RPSA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-RPSA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of paraffin-embedded mouse brain tissue using anti-RPSA antibody. Counter stained with hematoxylin.

Product Discontinued

RPSA Antibody (JF0955) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human 67kDa Laminin Receptor aa 250-295 / 295. (SwissProt: P08865 Human; SwissProt: P14206 Mouse; SwissProt: P38983 Rat)
• Localization: Cell membrane, Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPSA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RPSA Antibody (JF0955)

• 37 kDa laminin receptor precursor
• 37/67 kDa laminin receptor
• 37LRPNEM/1CHD4
• 67 kDa laminin receptor
• 67kD, ribosomal protein SA
• 67LR
• Colon carcinoma laminin-binding protein
• LAMBR37 kDa laminin receptor
• laminin binding protein
• laminin receptor 1 (67kD, ribosomal protein SA)
• Laminin receptor 1
• Laminin-binding protein precursor p40
• LAMR 1
• lamR
• LAMR140S ribosomal protein SA
• LBP
• LBP/p40
• LRP
• LRP/LR
• Multidrug resistance-associated protein MGr1-Ag
• p40
• ribosomal protein SA

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPSA