ROBO2 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ROBO2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROBO2 isoform 1
  Ser22-Ala312, RPVA, Pro313-Pro859
  Accession # Q9HCK4
• Specificity: Detects human ROBO2 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant rat ROBO1 and recombinant human ROBO4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO2 Antibody [Biotin]

• Drosophila) homolog 2
• ROBO2
• roundabout, axon guidance receptor, homolog 2 (Drosophila)
• SAX3

Background

Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31-504) and three fibronectin (FN) type III domains (aa 522-826). The cytoplasmic region contains multiple 15-20 aa long CC (conserved cytoplasmic) motifs (C0-C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186-1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22-285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD is 98% aa identical to the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1-ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).

1. Rajagopalan, S. et al. (2000) Neuron 28:767.
2. Guthrie, S. (2004) Curr. Biol. 14:R632.
3. Guthrie, S. (2001) Curr. Biol. 11:R300.
4. Seeger, M. et al. (1993) Neuron 10:409.
5. Kidd, T. et al. (1998) Cell 92:205.
6. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
7. Bashaw, G.J. et al. (2000) Cell 101:703.
8. Jen, J.C. et al. (2004) Science 304:1509.
9. GenBank Accession # Q9KCK4.
10. Isogai, T. et al. (2002) GenBank Accession # AK074780.
11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
12. Long, H. et al. (2004) Neuron 42:213.
13. Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534.
14. Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ROBO2
• Uniprot:
  • Q9HCK4()