ROBO2 Antibody

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ROBO2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROBO2
  Ser22-Ala312, RPVA, Pro313-Pro859
  Accession # Q9HCK4
• Specificity: Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO2 Antibody

• Drosophila) homolog 2
• ROBO2
• roundabout, axon guidance receptor, homolog 2 (Drosophila)
• SAX3

Background

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROBO2 Antibody (AF3147)(6)

Publications using AF3147

• M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord Cell Rep, 2019;26(12):3298-3312.e4. 2019 [PMID: 30893602] (Immunoprecipitation, Mouse)
• Geutskens SB, Hordijk PL, van Hennik PB The chemorepellent Slit3 promotes monocyte migration. J. Immunol., 2010;185(12):7691-8. 2010 [PMID: 21078908] (Flow Cytometry, Human)
• Takeuchi H, Inokuchi K, Aoki M Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb. Cell, 2010;141(6):1056-67. 2010 [PMID: 20550939] (IHC, Mouse)
• Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord. J. Neurosci., 2008;28(35):8698-708. 2008 [PMID: 18753371] (IHC-Fr, Chicken)
• Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons. J. Neurosci., 2008;28(16):4244-9. 2008 [PMID: 18417704] (IHC, Mouse)
• Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons. Dev. Biol., 2007;313(2):648-58. 2007 [PMID: 18054781] (IHC, Mouse)

Bioinformatics

• Gene Symbol: ROBO2
• Uniprot:
  • Q9HCK4()