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![Western Blot: ROBO1 Knockout A549 Cell Lysate [NBP3-18631] - Lane 1: Opal Prestained MW Marker. Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental. Lane 9: MCF-7 WCL. Load: 35ug lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 ~181kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ROBO1-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18631-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ROBO1 Knockout A549 Cell Lysate [NBP3-18631] - Lane 1: Opal Prestained MW Marker. Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental. Lane 9: MCF-7 WCL. Load: 35ug lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 ~181kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: Robo1 Overexpression Lysate (Adult Normal) [NBP2-09969] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Robo1.](http://images.novusbio.com/fullsize/Robo1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09969-img0001.jpg "Western Blot: Robo1 Overexpression Lysate (Adult Normal) [NBP2-09969] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Robo1.")
![Western Blot: Robo1 Overexpression Lysate (Adult Normal) [NBP2-09764] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Robo1.](http://images.novusbio.com/fullsize/Robo1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09764-img0001.jpg "Western Blot: Robo1 Overexpression Lysate (Adult Normal) [NBP2-09764] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Robo1.")
![Western Blot: Robo1 Overexpression Lysate (Adult Normal) [NBP2-05697] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Robo1.](http://images.novusbio.com/fullsize/Robo1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05697-img0001.jpg "Western Blot: Robo1 Overexpression Lysate (Adult Normal) [NBP2-05697] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Robo1.")