TSG Antibody

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TSG was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) overnight at 4 °C. Tissue was stained (red) and ...read more
TSG was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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TSG Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSG
Cys25-Phe222
Accession # Q9EP52
Specificity
Detects mouse TSG in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TWSG1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publication using
AF756 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSG Antibody

  • TSG
  • TSGtwisted gastrulation protein homolog 1
  • twisted gastrulation homolog 1 (Drosophila)
  • TWSG1

Background

Twisted Gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning by regulating BMP signaling in Drosophila and Xenopus. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. Mouse TSG encodes a 222 amino acid (aa) residue precursor protein with a 24 aa residue putative signal peptide that is cleaved to generate the 198 aa residue mature protein. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. dTSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. In vivo, TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.

  1. Mason, et al. (1994) Genes Dev. 8:1489.
  2. Oelgeschlager, et al. (2000) Nature 405:757.
  3. Yu, et al. (2000) Development 127:2143.
  4. Dale (2000) Current Biology 10:R671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TSG Antibody (AF756)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF756

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Bioinformatics Tool for TSG Antibody (AF756)

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Bioinformatics

Gene Symbol TWSG1
Uniprot