TSG was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) overnight at 4 °C. Tissue was stained (red) and ...read more
TSG was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TSG Antibody
TSGtwisted gastrulation protein homolog 1
twisted gastrulation homolog 1 (Drosophila)
Twisted Gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning by regulating BMP signaling in Drosophila and Xenopus. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. Mouse TSG encodes a 222 amino acid (aa) residue precursor protein with a 24 aa residue putative signal peptide that is cleaved to generate the 198 aa residue mature protein. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. dTSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. In vivo, TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.
Mason, et al. (1994) Genes Dev. 8:1489.
Oelgeschlager, et al. (2000) Nature 405:757.
Yu, et al. (2000) Development 127:2143.
Dale (2000) Current Biology 10:R671.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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