RNASE7 Antibody (CL0223) - Azide and BSA Free

Analysis in human skin and liver tissues using NBP3-43952 antibody. Corresponding RNASE7 RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)]Lane 3: RNASE7 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Staining of human tonsil shows moderate to strong cytoplasmic positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0223
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RNASE7 Antibody (CL0223) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RNASE7 Antibody (CL0223) - Azide and BSA Free (NBP2-52883) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9H1E1, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNASE7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for RNASE7 Antibody (CL0223) - Azide and BSA Free

• EC 3.1.27
• EC 3.1.27.-
• EC 3.1.27.5
• MGC133220
• ribonuclease 7
• ribonuclease, RNase A family, 7
• RNase 7
• SAP-2
• Skin-derived antimicrobial protein 2

Background

RNASE7 is a member of the RNase A superfamily that has broad-spectrum antimicrobial activity and ribonuclease activity (Harder and Schroder, 2002 [PubMed 12244054]; Zhang et al., 2003 [PubMed 12527768])

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNASE7