Ribonuclease A Antibody

Images

 
Western Blot: Ribonuclease A Antibody [NBP1-87804] - Analysis in human pancreas tissue.
Immunocytochemistry/ Immunofluorescence: Ribonuclease A Antibody [NBP1-87804] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining in human pancreas and prostate tissues using NBP1-87804 antibody. Corresponding RNASE1 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Ribonuclease A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNASE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Ribonuclease A Protein (NBP1-87804PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ribonuclease A Antibody

  • EC 3.1.27.5
  • HP-RNase
  • MGC12408
  • RIB1
  • RIB-1
  • Ribonuclease 1
  • Ribonuclease A
  • ribonuclease pancreatic
  • ribonuclease, RNase A family, 1 (pancreatic)
  • RNase A
  • RNase UpI-1
  • RNS1RNase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNASE1