Ribonuclease A Antibody - BSA Free

Western Blot: Ribonuclease A Antibody [NBP1-87804] - Analysis in human pancreas tissue.

Immunocytochemistry/Immunofluorescence: Ribonuclease A Antibody [NBP1-87804] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles.

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining in human pancreas and prostate tissues using NBP1-87804 antibody. Corresponding RNASE1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human prostate shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ribonuclease A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ribonuclease A Antibody - BSA Free (NBP1-87804) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNASE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Ribonuclease A Protein (NBP1-87804PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Ribonuclease A Antibody - BSA Free

• EC 3.1.27.5
• HP-RNase
• MGC12408
• RIB1
• RIB-1
• Ribonuclease 1
• Ribonuclease A
• ribonuclease pancreatic
• ribonuclease, RNase A family, 1 (pancreatic)
• RNase A
• RNase UpI-1
• RNS1RNase 1

Background

The Ribonuclease A gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNASE1