RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007)

Images

 
Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were ...read more
Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S5p and optimized PCR primer pairs for ...read more
Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in ...read more
ELISA: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Cross reactivity of the antibody directed against Pol IIS5p. To test the specificity an ELISA was performed using a serial dilution of the ...read more
Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody ...read more

Product Details

Summary
Reactivity Hu, ZeSpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF, CHIP-SEQ, KD
Clone
C.15200007
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) Summary

Immunogen
The exact immunogen is propietary information.
Modification
p Ser5
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POLR2A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation Sequencing 1 - 2 ug/ChIP
  • Chromatin Immunoprecipitation 1 - 2 ug/IP
  • ELISA 1:3000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Knockdown Validated
  • Western Blot 1:1000
Reviewed Applications
Read 1 Review rated 4
using
NBP2-59219 in the following applications:

Publications
Read Publication using
NBP2-59219 in the following applications:

Reactivity Notes

Use in Zebrafish reported in secitific publication PMID: 32652076

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007)

  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II subunit RPB1
  • DNA-directed RNA polymerase III largest subunit
  • EC 2.7.7.48
  • EC 2.7.7.6
  • hRPB220
  • hsRPB1
  • MGC75453
  • POLR2
  • POLR2A
  • POLRA
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • RNA Pol 2
  • RNA Polymerase 2
  • RNA polymerase II subunit B1
  • RNA Polymerase II
  • RNA-directed RNA polymerase II subunit RPB1
  • RNAPII
  • RPB1
  • RPBh1
  • RpIILS
  • RPO2
  • RPOL2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNA Polymerase II/POLR2A Antibody (NBP2-59219)(1)

We have publications tested in 1 confirmed species: Zebrafish.

We have publications tested in 1 application: ChIP.


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(1)
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Review for RNA Polymerase II/POLR2A Antibody (NBP2-59219) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-59219:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation RNA Polymerase II/POLR2A NBP2-59219
Enlarge
4
reviewed by:
Wendy Lyu
ChIP Human 09/11/2020
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHuman breast cancer cell lysate (MCF-7),T47D human breast cancer cell line
SpeciesHuman
Lot001-12

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Video Protocols

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ICC/IF Video Protocol

FAQs for RNA Polymerase II/POLR2A Antibody (NBP2-59219) (0)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Wendy Lyu
09/11/2020
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol POLR2A