RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

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Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Western blot analysis of extracts of various cell lines, using RNA Polymerase II/POLR2A Rabbit mAb (NBP3-16910) at 1:1000 dilution.MCF7 ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of A-549 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of PC-12 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of NIH/3T3 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of HeLa cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using RNA Polymerase II/POLR2A Rabbit mAb ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of PC-12 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using RNA Polymerase II/POLR2A Rabbit mAb at ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RNA Polymerase II/POLR2A Rabbit mAb at ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of NIH/3T3 cells using RNA Polymerase II/POLR2A Rabbit mAb at ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RNA Polymerase II/POLR2A Rabbit mAb at ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using RNA Polymerase II/POLR2A Rabbit mAb ...read more
Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of HeLa cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution ...read more
Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of A-549 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
10C7U2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A phospho specific peptide corresponding to residues surrounding S5 of human RNA Polymerase II/POLR2A (P24928).
Modification
p Ser5
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
POLR2A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II subunit RPB1
  • DNA-directed RNA polymerase III largest subunit
  • EC 2.7.7.48
  • EC 2.7.7.6
  • hRPB220
  • hsRPB1
  • MGC75453
  • POLR2
  • POLR2A
  • POLRA
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • RNA Pol 2
  • RNA Polymerase 2
  • RNA polymerase II subunit B1
  • RNA Polymerase II
  • RNA-directed RNA polymerase II subunit RPB1
  • RNAPII
  • RPB1
  • RPBh1
  • RpIILS
  • RPO2
  • RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLR2A