Rif1 Antibody

Images

 
Western Blot: Rif1 Antibody [NB100-1587] - Detection of Human Rif1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified ...read more
Immunohistochemistry: Rif1 Antibody [NB100-1587] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Rif1 Antibody [NB100-1587] - Whole cell lysate from 293T cells. RIF1 was also immunoprecipitated using rabbit anti-RIF1 antibody NB100-1586.
Western Blot: Rif1 Antibody [NB100-1587] - Whole cell lysate (50 ug) from HeLa cells. Antibody used at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1587] - Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Rif1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2425 and the C-terminus (residue 2472) of human Rap1 Interacting Factor 1 using the numbering given in entry NP_060621.3 (GeneID 55183).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rif1 Antibody

  • DKFZp434D1026
  • DKFZp781N1478
  • FLJ10599
  • FLJ12870
  • RAP1 interacting factor homolog (yeast)
  • Rap1-interacting factor 1 homolog
  • telomere-associated protein RIF1

Background

RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to DNA damage is completely dependent on ATM. Alternate names for RIF1 include telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, FLJ12870, and DKFZp781N1478.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Rif1 NB100-1587

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Blogs on Rif1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

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Bioinformatics

Gene Symbol RIF1
Entrez
Uniprot