CLEC10A/CD301 Antibody (ER-MP23) - BSA Free

Immunohistochemistry: CLEC10A/CD301 Antibody (ER-MP23) [NB100-64874] - Staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). High power.

Flow Cytometry: CLEC10A/CD301 Antibody (ER-MP23) [NB100-64874] - A surface stain was performed on RAW 246.7 cells with CLEC10A/CD301 antibody (ER-MP23) NB100-64874AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunohistochemistry: CLEC10A/CD301 Antibody (ER-MP23) [NB100-64874] - Staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Low power

Immunohistochemistry: CLEC10A/CD301 Antibody (ER-MP23) [NB100-64874] - Staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Medium power

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: ER-MP23
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CLEC10A/CD301 Antibody (ER-MP23) - BSA Free Summary

• Immunogen: Balb/c macrophage precursor cell hybrids
• Localization: Membrane; Single pass type II membrane protein.
• Specificity: NB100-64874 recognizes murine CD301, a 38 kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated. Clone ER-MP23 is reported to block the function of mouse CD301. The antibody binds both MGL1 and MGL2 homologues.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CLEC10A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CLEC10A/CD301 Antibody (ER-MP23) - BSA Free

• CD301 antigen
• CD301
• CD301C-type lectin domain family 10 member A
• CLEC10A
• CLECSF13
• CLECSF14
• CLECSF14macrophage C-type lectin
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived)
• C-type lectin domain family 10, member A
• C-type lectin superfamily member 14
• HML2
• HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived)
• macrophage lectin 2 (calcium dependent)
• Macrophage lectin 2
• MGL

Background

CLEC10A (C type lectin domain family 10, member A) also known as CD301 or macrophage galactose N-acetylgalactosamine lectin (MGL), is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Family members share a common protein fold and have diverse functions which include; cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 is reported to block the function of mouse CD301.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC10A/CD301 Antibody (NB100-64874)(3)

Publications using NB100-64874

• Luan D, Dadpey B, Zaid J et al. Adipocyte-Secreted IL-6 Sensitizes Macrophages to IL-4 Signaling Diabetes 2023-03-01 [PMID: 36449000] (FLOW, Mouse)
• Eggink L, Hoober J Resolution of Eczema with Multivalent Peptides JID Innovations 2022-07-01 [PMID: 36039327]
• Brooks PJ CD301/CLEC10 Expression and Its Role in Il-4 Driven Multinucleated Giant Cell Formation Thesis 2020-01-01 [PMID: 33571108]

Bioinformatics

• Gene Symbol: CLEC10A
• Entrez:
  • 17312(Mouse)