ISCU Antibody

Images

 
Western Blot: ISCU Antibody [NBP2-14998] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: ISCU Antibody [NBP2-14998] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ISCU protein stained by ISCU antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. ...read more
Immunohistochemistry-Paraffin: ISCU Antibody [NBP2-14998] - Paraffin-embedded mouse muscle. ISCU antibody [N1C3] dilution: 1:500.
Western Blot: ISCU Antibody [NBP2-14998] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: ISCU Antibody [NBP2-14998] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ISCU Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ISCU. The exact sequence is proprietary.
Predicted Species
Rat (97%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ISCU
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Drosophila (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ISCU Antibody

  • 2310020H20Rik
  • HML
  • hnifU
  • iron-sulfur cluster assembly enzyme ISCU, mitochondrial
  • iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog
  • IscU
  • ISU2
  • MGC74517
  • NIFU
  • NifU-like N-terminal domain containing
  • NifU-like N-terminal domain-containing protein
  • NifU-like protein
  • NIFUN

Background

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ISCU
Uniprot