Biological Strategies: Western Blot: Rif1 Antibody [NB100-1586] - Detection of Human RIF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1586] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. ...read more
Independent Antibodies: Immunoprecipitation: Rif1 Antibody [NB100-1586] - Whole cell lysate from HeLa cells. Whole cell lysate from 293T cells. Affinity purified rabbit anti-RIF1 antibody NB100-1586. RIF1 was ...read more
Rif1 Antibody [NB100-1586] - Detection of human RIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. ...read more
RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to DNA damage is completely dependent on ATM. Alternate names for RIF1 include telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, FLJ12870, and DKFZp781N1478.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post.
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