Rif1 Antibody

Images

 
Biological Strategies: Western Blot: Rif1 Antibody [NB100-1586] - Detection of Human RIF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1586] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. ...read more
Independent Antibodies: Immunoprecipitation: Rif1 Antibody [NB100-1586] - Whole cell lysate from HeLa cells. Whole cell lysate from 293T cells. Affinity purified rabbit anti-RIF1 antibody NB100-1586. RIF1 was ...read more
Rif1 Antibody [NB100-1586] - Detection of human RIF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Rif1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1425 and 1450 of human Rap1 Interacting Factor 1 using the numbering given in entry NP_060621.3 (GeneID 55183).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1-5 ug/mg of lysate
  • Western Blot 1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-1586 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rif1 Antibody

  • DKFZp434D1026
  • DKFZp781N1478
  • FLJ10599
  • FLJ12870
  • RAP1 interacting factor homolog (yeast)
  • Rap1-interacting factor 1 homolog
  • telomere-associated protein RIF1

Background

RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to DNA damage is completely dependent on ATM. Alternate names for RIF1 include telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, FLJ12870, and DKFZp781N1478.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rif1 Antibody (NB100-1586)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Rif1 Products

Array NB100-1586

Blogs on Rif1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

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Bioinformatics

Gene Symbol RIF1
Entrez
Uniprot