RGM-B Antibody (398528)

• Reactivity: Hu
• Applications: WB
• Clone: 398528
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

RGM-B Antibody (398528) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RGM-B isoform 1
  Gly46-Ser411
  Accession # Q6NW40
• Specificity: Detects human RGM-B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse RGM-B, recombinant human (rh) RGM-A, or rhRGM-C is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RGMB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RGM-B Antibody (398528)

• DKFZp434P228
• Dragon
• DRG11-responsive axonal guidance and outgrowth of neurite
• RGM domain family, member B
• RGMB
• RGM-B

Background

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 45 amino acid (aa) signal sequence, a 368 aa mature region, and a 24 aa C-terminal prosegment (3). RGM-B contains an RGD motif, two potential N-linked glycosylation sites, and an abbreviated von Willebrand factor domain. There is a potential proteolytic cleavage site within the VWF domain (4). Alternative splicing may generate isoforms of RGM-B with N-terminal extensions or truncation following the VWF domain. Mature human RGM-B shares 52% and 36% aa sequence identity with the comparable regions of RGM-A and RGM-C, respectively. It shares 98%, 92%, 92%, and 78% aa sequence identity with macaque, mouse, bovine, and chicken RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3‑5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2‑5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

1. Monnier, P.P. et al. (2002) Nature 419:392.
2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGM-B Antibody (MAB3630)(1)

Publication using MAB3630

• Xiao , Yanping, Yu , Sanhong, Zhu , Baogong, Bedoret , Denis, Bu , Xia, Francisco , Loise M, Hua , Ping, Duke-Cohan , Jonathan, Umetsu , Dale T, Sharpe , Arlene H, DeKruyff , Rosemari, Freeman , Gordon J RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 2014-04-21;211(5):943-59. 2014-04-21 [PMID: 24752301] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: RGMB
• Uniprot:
  • Q6NW40()