RGM-B Antibody (398528) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 398528
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

RGM-B Antibody (398528) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RGM-B isoform 1
  Gly46-Ser411
  Accession # Q6NW40
• Specificity: Detects human RGM-B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse RGM-B, recombinant human (rh) RGM-A, or rhRGM-C is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RGM-B Antibody (398528) [Alexa Fluor® 647]

• DKFZp434P228
• Dragon
• DRG11-responsive axonal guidance and outgrowth of neurite
• RGM domain family, member B
• RGMB
• RGM-B

Background

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 45 amino acid (aa) signal sequence, a 368 aa mature region, and a 24 aa C-terminal prosegment (3). RGM-B contains an RGD motif, two potential N-linked glycosylation sites, and an abbreviated von Willebrand factor domain. There is a potential proteolytic cleavage site within the VWF domain (4). Alternative splicing may generate isoforms of RGM-B with N-terminal extensions or truncation following the VWF domain. Mature human RGM-B shares 52% and 36% aa sequence identity with the comparable regions of RGM-A and RGM-C, respectively. It shares 98%, 92%, 92%, and 78% aa sequence identity with macaque, mouse, bovine, and chicken RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3‑5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2‑5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.