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RGMB Products By Gene Name

5 Results for "RGMB"

AF3597

RGM-B Antibody [Unconjugated]

(6 Publications)

100 μg

AF3597

RGM-B was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglia) using 1.7 µg/mL Sheep Anti-Mouse RGM-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3597) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Subregion-specific expression of RGMs and Neogenin in the hippocampus.In situ hybridization (A–C, J–L) and immunohistochemistry (D–I, M–O) on coronal mouse brain sections at E16.5 (A–I) and P5 (J–O). Sections in D–I and M–O are counterstained in blue with fluorescent Nissl. (A–F) RGMa mRNA and protein are expressed in the ventricular zone (VZ), dentate gyrus (DG) and cornu ammonis (CA) region. Strong expression of RGMb mRNA and protein is detected in the pial surface lining the hippocampal fissure (HF). Neogenin transcripts and protein are widely expressed in the developing hippocampus (Hip). (G–I) Immunostaining with isotype matched controls. (J–L) In situ hybridization at P5 shows strong but differential expression patterns of RGMa, RGMb and Neogenin in the CA pyramidal cell layers (Pyr). In addition, strong expression of Neogenin is detected in the granular layer (GC) of the DG. (M–O) Immunohistochemistry reveals expression of RGMa and weak expression of RGMb in the stratum lacunosum moleculare (SLM) and fimbria (FIM). Neogenin strongly labels different hippocampal layers. CX, cortex; Hb, habenula; HC, hippocampal commissure; PO, polymorph layer; SO, stratum oriens; SR, stratum radiatum; Th, thalamus. Scale bar A–C: 400 µm, D–F: 300 µm, G–I: 300 µm, J–L: 500 µm and M–O: 400 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differential expression of RGMs and Neogenin in the cerebellum.In situ hybridization (A–C, G–I, M–O) and immunohistochemistry (D–F, J–L, P–R) on coronal mouse brain sections at E16.5 (A–F), P5 (G–L) and in the adult (M–R). (A–I, M–O) In situ hybridization and immunohistochemistry reveals strong and broad expression of RGMa, RGMb and Neogenin in the cerebellum (CB). (J–L) Immunostaining shows expression of RGMa and Neogenin in all cerebellar layers at P5. RGMb is expressed in the internal granular layer (IGL), Purkinje cell layer (PCL) and external granular layer (EGL). (P–R) In the adult, RGMa, RGMb and Neogenin protein are expressed in Purkinje cells (PCs) and axons in the granular cell layer (GCL), PCL and molecular layer (ML). Neogenin strongly labels PC dendrites in the ML. DCN, deep cerebellar nuclei; WM, white matter; VZ, ventricular zone. Scale bar A–C: 300 µm, D–F: 250 µm, G–I: 150 µm, J–L: 150 µm, M–O: 100 µm and P–R: 50 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

RGMa, RGMb and Neogenin display partially complementary patterns of expression in the developing cortex and on cortical projections.In situ hybridization (A–C′) and immunohistochemistry (D–L) on coronal mouse brain sections at E16.5. Panels A′–C′ and D′–F′ show higher magnifications of the boxed areas in A–C and D–F, respectively. Sections in D–L are counterstained in blue with fluorescent Nissl. (A–C′) In situ hybridization reveals strong expression of RGMa and Neogenin, and moderate expression of RGMb, in the embryonic mouse cortex. Arrow in A indicates neurons of the lateral migratory stream. (D–I) RGMa and Neogenin protein are strongly expressed in the cortex and on various cortical axon projections. Strong staining for RGMb (E), and Neogenin (F), is detected in the corpus callosum (CC). The internal capsule (IC) stains strongly for RGMa (G). (J–L) Immunostaining with isotype-matched control antibodies did show significant staining. CP, cortical plate; FIM, fimbria; IZ, intermediate zone; LV, lateral ventricle; MZ, marginal zone; SP, subplate; STR, striatum; SVZ, subventricular zone; VZ, ventricular zone. Scale bar A–C 400 µm, A′–C′ 200 µm, D–F 300 µm, D′–F′ 150 µm, G-I 300 µm and J-M 300 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3597U-100UG
Alexa Fluor 405	AF3597V-100UG
Alexa Fluor 488	AF3597G-100UG
Alexa Fluor 532	AF3597X-100UG
Alexa Fluor 594	AF3597T-100UG
Alexa Fluor 647	AF3597R-100UG
Alexa Fluor 700	AF3597N-100UG
Alexa Fluor 750	AF3597S-100UG
Alexa Fluor Plus 405	AF3597AFP405-100UG
Alexa Fluor Plus 488	AF3597AFP488-100UG
Alexa Fluor Plus 555	AF3597AFP555-100UG
Alexa Fluor Plus 594	AF3597AFP594-100UG
Alexa Fluor Plus 647	AF3597AFP647-100UG
Alexa Fluor Plus 680	AF3597AFP680-100UG
Allophycocyanin	AF3597A
Biotin	BAF3597
CoraFluor 1	AF3597CL1
DyLight 350	AF3597D
DyLight 405	AF3597E
DyLight 488	AF3597K
DyLight 550	AF3597L
DyLight 594	AF3597M
DyLight 650	AF3597W
DyLight 680	AF3597Y
DyLight 755	AF3597Z
FITC	AF3597F
HRP	AF3597H
Janelia Fluor 525	AF3597JF525
Janelia Fluor 549	AF3597I
Janelia Fluor 646	AF3597J
Janelia Fluor 669	AF3597JF669
PE	AF3597P
PerCP	AF3597C
mFluor Violet 450 SE	AF3597MFV450
mFluor Violet 500 SE	AF3597MFV500
mFluor Violet 610 SE	AF3597MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3597-SP

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AF3630

RGM-B Antibody [Unconjugated]

(4 Publications)

100 μg

AF3630

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

A172 human glioblastoma cell line was stained with Goat Anti-Human RGM-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3630, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." alt="A172 human glioblastoma cell line was stained with Goat Anti-Human RGM-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3630, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3630U-100UG
Alexa Fluor 405	AF3630V-100UG
Alexa Fluor 488	AF3630G-100UG
Alexa Fluor 532	AF3630X-100UG
Alexa Fluor 594	AF3630T-100UG
Alexa Fluor 647	AF3630R-100UG
Alexa Fluor 700	AF3630N-100UG
Alexa Fluor 750	AF3630S-100UG
Alexa Fluor Plus 405	AF3630AFP405-100UG
Alexa Fluor Plus 488	AF3630AFP488-100UG
Alexa Fluor Plus 555	AF3630AFP555-100UG
Alexa Fluor Plus 594	AF3630AFP594-100UG
Alexa Fluor Plus 647	AF3630AFP647-100UG
Alexa Fluor Plus 680	AF3630AFP680-100UG
Allophycocyanin	AF3630A
Biotin	AF3630B
CoraFluor 1	AF3630CL1
DyLight 350	AF3630D
DyLight 405	AF3630E
DyLight 488	AF3630K
DyLight 550	AF3630L
DyLight 594	AF3630M
DyLight 650	AF3630W
DyLight 680	AF3630Y
DyLight 755	AF3630Z
FITC	AF3630F
HRP	AF3630H
Janelia Fluor 525	AF3630JF525
Janelia Fluor 549	AF3630I
Janelia Fluor 646	AF3630J
Janelia Fluor 669	AF3630JF669
PE	AF3630P
PerCP	AF3630C
mFluor Violet 450 SE	AF3630MFV450
mFluor Violet 500 SE	AF3630MFV500
mFluor Violet 610 SE	AF3630MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3630-SP

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3597-RG

Recombinant Mouse RGM-B Protein, CF

(3 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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3630-RG

Recombinant Human RGM-B Protein, CF

(2 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB3630

RGM-B Antibody (398528)

(1 Publication)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3630U-100UG
Alexa Fluor 405	FAB3630V-100UG
Alexa Fluor 488	FAB3630G-100UG
Alexa Fluor 532	FAB3630X-100UG
Alexa Fluor 594	FAB3630T-100UG
Alexa Fluor 647	FAB3630R-100UG
Alexa Fluor 700	FAB3630N-100UG
Alexa Fluor 750	FAB3630S-100UG
Alexa Fluor Plus 405	FAB3630AFP405-100UG
Alexa Fluor Plus 488	FAB3630AFP488-100UG
Alexa Fluor Plus 555	FAB3630AFP555-100UG
Alexa Fluor Plus 594	FAB3630AFP594-100UG
Alexa Fluor Plus 647	FAB3630AFP647-100UG
Alexa Fluor Plus 680	FAB3630AFP680-100UG
Allophycocyanin	FAB3630A
Biotin	FAB3630B
CoraFluor 1	FAB3630CL1
DyLight 350	FAB3630D
DyLight 405	FAB3630E
DyLight 488	FAB3630K
DyLight 550	FAB3630L
DyLight 594	FAB3630M
DyLight 650	FAB3630W
DyLight 680	FAB3630Y
DyLight 755	FAB3630Z
FITC	FAB3630F
HRP	FAB3630H
Janelia Fluor 525	FAB3630JF525
Janelia Fluor 549	FAB3630I
Janelia Fluor 646	FAB3630J
Janelia Fluor 669	FAB3630JF669
PE	FAB3630P
PerCP	FAB3630C
mFluor Violet 450 SE	FAB3630MFV450
mFluor Violet 500 SE	FAB3630MFV500
mFluor Violet 610 SE	FAB3630MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3630-SP

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