RFC4 Antibody - BSA Free

Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.

Immunohistochemistry: RFC4 Antibody [NBP2-49283] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human kidney shows low expression as expected.

Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human testis shows high expression.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RFC4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RFC4 Antibody - BSA Free (NBP2-49283) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: VVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  RFC4 Recombinant Protein Antigen (NBP2-49283PEP)

Reactivity Notes

Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RFC4 Antibody - BSA Free

• A1 37 kDa subunit
• A1
• Activator 1 37 kDa subunit
• Activator 1 subunit 4
• MGC27291
• replication factor C (activator 1) 4, 37kDa
• Replication factor C 37 kDa subunit
• replication factor C subunit 4
• RFC 37 kDa subunit
• RF-C 37 kDa subunit
• RFC37replication factor C (activator 1) 4 (37kD)

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFC4