| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73859 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48927-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38162-20ul | |||
![Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Western-Blot-NBP2-45946-img0005.jpg "Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.")
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunofluorescence-NBP2-45946-img0001.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunohistochemistry-NBP2-45946-img0004.jpg)
![Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of extracts (35ug) from 9 different cell lines by using RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Western-Blot-NBP2-45946-img0006.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunohistochemistry-NBP2-45946-img0002.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunohistochemistry-NBP2-45946-img0003.jpg)
![Western Blot: RFC4 Antibody [NBP2-48927] - Analysis using Anti-RFC4 antibody NBP2-48927 (A) shows similar pattern to independent antibody NBP2-49283 (B).](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-48927-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RFC4 Antibody [NBP2-48927] - Analysis using Anti-RFC4 antibody NBP2-48927 (A) shows similar pattern to independent antibody NBP2-49283 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-48927] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48927-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0007.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining of human Staining of human lymph node. shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0002.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0005.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33083-img0004.jpg "Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - RFC4 antibody detects RFC4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-231xenograft. RFC4 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0008.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP1-33083-img0006.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0005.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (NBP1-33083) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33083_rabbit-polyclonal-rfc4-antibody-910202420134095.jpg)
![Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-233-img0054.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0011.jpg "Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Immunohistochemistry-Paraffin-H00005984-M01-img0007.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0008.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - RFC4 monoclonal antibody (M01), clone 1C12 Analysis of RFC4 expression in K-562.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0010.jpg)
![Sandwich ELISA: RFC4 Antibody (1C12) [H00005984-M01] - Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Sandwich-ELISA-H00005984-M01-img0009.jpg)
![Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-49283-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0005.jpg)
![Immunohistochemistry: RFC4 Antibody [NBP2-49283] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-NBP2-49283-img0001.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0003.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0004.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RFC4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97936-img0002.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-91220241519565.jpg "Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: RFC4 Antibody [NBP3-38162] - Immunoprecipitation analysis of 200 ug extracts of K562 cells using RFC4 antibody. Western blot was performed from the immunoprecipitate using RFC4 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202414201340.jpg)
![Western Blot: RFC4 Antibody [NBP3-38162] - Western blot analysis of various lysates using RFC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202415143626.jpg)
![Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202415175266.jpg)
![SDS-Page: Recombinant Human RFC4 Protein [H00005984-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005984-P01-1.jpg "SDS-Page: Recombinant Human RFC4 Protein [H00005984-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-403-img0001.jpg "Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.")
![Western Blot: RFC4 Overexpression Lysate (Adult Normal) [NBL1-15294] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RFC4.](http://images.novusbio.com/fullsize/RFC4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15294-img0002.jpg "Western Blot: RFC4 Overexpression Lysate (Adult Normal) [NBL1-15294] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RFC4.")
![Western Blot: RFC4 Overexpression Lysate (Adult Normal) [NBP2-04913] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RFC4.](http://images.novusbio.com/fullsize/RFC4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04913-img0001.jpg "Western Blot: RFC4 Overexpression Lysate (Adult Normal) [NBP2-04913] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RFC4.")