RFC4 Antibodies
We offer RFC4 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each RFC4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RFC4 Antibodies can be used in a variety of model species: Canine, Human, Monkey, Mouse, Rat. Use the list below to choose the RFC4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RFC4 polyclonal antibodies and browse our RFC4 monoclonal antibody catalog.
Alternate Names for RFC4 Antibodies
anti-RFC4 antibody, anti-A1 37 kDa subunit antibody, anti-A1 antibody, anti-Activator 1 37 kDa subunit antibody, anti-Activator 1 subunit 4 antibody, anti-MGC27291 antibody, anti-replication factor C (activator 1) 4, 37kDa antibody, anti-Replication factor C 37 kDa subunit antibody, anti-replication factor C subunit 4 antibody, anti-RF-C 37 kDa subunit antibody, anti-RFC 37 kDa subunit antibody, anti-RFC37replication factor C (activator 1) 4 (37kD) antibody
11 Results for "rfc4" in Primary Antibodies
![Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Western-Blot-NBP2-45946-img0005.jpg "Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.")
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunofluorescence-NBP2-45946-img0001.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunohistochemistry-NBP2-45946-img0004.jpg)
![Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of extracts (35ug) from 9 different cell lines by using RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Western-Blot-NBP2-45946-img0006.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunohistochemistry-NBP2-45946-img0002.jpg)
![Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RFC4-Antibody-OTI1A8-Immunohistochemistry-NBP2-45946-img0003.jpg)
![Western Blot: RFC4 Antibody [NBP2-48927] - Analysis using Anti-RFC4 antibody NBP2-48927 (A) shows similar pattern to independent antibody NBP2-49283 (B).](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-48927-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RFC4 Antibody [NBP2-48927] - Analysis using Anti-RFC4 antibody NBP2-48927 (A) shows similar pattern to independent antibody NBP2-49283 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-48927] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48927-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0007.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining of human Staining of human lymph node. shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0002.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0005.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33083-img0004.jpg "Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - RFC4 antibody detects RFC4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-231xenograft. RFC4 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0008.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP1-33083-img0006.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0005.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (NBP1-33083) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33083_rabbit-polyclonal-rfc4-antibody-910202420134095.jpg)
![Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-233-img0054.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0011.jpg "Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Immunohistochemistry-Paraffin-H00005984-M01-img0007.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0008.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - RFC4 monoclonal antibody (M01), clone 1C12 Analysis of RFC4 expression in K-562.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0010.jpg)
![Sandwich ELISA: RFC4 Antibody (1C12) [H00005984-M01] - Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Sandwich-ELISA-H00005984-M01-img0009.jpg)
![Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-49283-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RFC4 Antibody [NBP2-49283] - Analysis using Anti-RFC4 antibody NBP2-49283 (A) shows similar pattern to independent antibody NBP2-48927 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0005.jpg)
![Immunohistochemistry: RFC4 Antibody [NBP2-49283] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-NBP2-49283-img0001.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0003.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-49283] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-49283-img0004.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RFC4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97936-img0002.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-91220241519565.jpg "Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: RFC4 Antibody [NBP3-38162] - Immunoprecipitation analysis of 200 ug extracts of K562 cells using RFC4 antibody. Western blot was performed from the immunoprecipitate using RFC4 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202414201340.jpg)
![Western Blot: RFC4 Antibody [NBP3-38162] - Western blot analysis of various lysates using RFC4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202415143626.jpg)
![Immunocytochemistry/ Immunofluorescence: RFC4 Antibody [NBP3-38162] - Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38162_rabbit-rfc4-pab-912202415175266.jpg)
![Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-403-img0001.jpg "Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.")