RelA/NFkB p65 [p Ser529] Antibody

Images

 
Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF ...read more
Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - 5 cells.
Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RelA/NFkB p65 [p Ser529] Antibody Summary

Description
This antibody was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen
RelA/NFkB p65 peptide corresponding to a region near [p Ser529] of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Modification
p Ser529
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal.
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Simple Western
  • ELISA 1:5000-1:25000
  • Immunohistochemistry 1:200-1:1000
Application Notes
Anti-phospho NFkB antibody reacts human pS529 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Simple Western reported by an internal validation. Separated by Charge, antibody dilution of 1:50.
Control Peptide
RelA/NFkB p65 Protein (NBP1-97000)
RelA/NFkB p65 Protein (NBP1-97001)
Publications
Read Publications using NBP1-77808.

Reactivity Notes

Cross reactivity with RelA/NFkB p65 [p Ser529] from other sources has not been tested.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
0.1% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for RelA/NFkB p65 [p Ser529] Antibody

  • MGC131774
  • NF kB p65 phospho
  • nf kb p65
  • NFkB p65 phospho
  • NF-kB p65 phospho
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • phospho NF kB p65
  • phospho NFkB p65
  • phospho NF-kB p65
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NBP1-77808)(6)

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Video Protocols

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Blogs on RelA/NFkB p65.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels...  Read full blog post.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez
Uniprot