RelA/NFkB p65 [p Ser276] Antibody

Images

 
Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF ...read more
Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The ...read more
Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.
Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 ...read more
Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
80 mg/ml

Order Details

RelA/NFkB p65 [p Ser276] Antibody Summary

Description
This antibody was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).Sequence information: QLRRPpSDRELSC
Modification
p Ser276
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:30000
  • Immunohistochemistry 1:200-1:800
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Phospho NFkB antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. A 1:500 dilution has been used for staining p65 in human kidney tissue by IHC. Tissue was formalin fixed and paraffin embedded. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. All conditions must be user optimized.
Control Peptide
RelA/NFkB p65 Protein (NBP1-97002)
RelA/NFkB p65 Protein (NBP1-97003)
Publications
Read Publications using NBP1-77807.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.01% Sodium Azide
Concentration
80 mg/ml
Purity
Delipidation and Defibrination

Alternate Names for RelA/NFkB p65 [p Ser276] Antibody

  • MGC131774
  • NF kB p65 phospho
  • nf kb p65
  • NFkB p65 phospho
  • NF-kB p65 phospho
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NBP1-77807)(4)

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez