Recombinant Rat TIM-1/KIM-1/HAVCR Protein, CF

Recombinant Rat TIM-1/KIM-1/HAVCR (Catalog # 3689-TM) inhibits anti-CD3-induced proliferation of stimulated human Tcells. Human T cells cultured for 72 hours with PHA were incubatedfor an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3and Recombinant Rat TIM-1/KIM-1/HAVCR. The ED50 for this effect is 0.4‑2 µg/mL.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat TIM-1/KIM-1/HAVCR Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and rrKIM-1.
  The ED₅₀ for this effect is 0.4‑2 µg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived rat TIM-1/KIM-1/HAVCR protein
  Ser18-Val238, with a C-terminal 6-His tag
• Accession #: O54947
• N-terminal Sequence: Ser18
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Havcr1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat TIM-1/KIM-1/HAVCR Protein, CF

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

KIM-1 (Kidney-injury molecule-1; also TIM-1 and HAVCR) is a 50‑80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules (1‑5). This gene family is involved in the regulation of Th1 and Th2‑cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1‑8) vs. only three genes in human (# 1, 3, 4) (1, 2, 5). It is unknown if the rat genome exactly parallels that of mouse. Rat KIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (4). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse KIM-1 gene is highly polymorphic and this may be reflected in rat (4, 6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule (7). A similar process has now been described in rat (8). The ECD of rat KIM-1 is 50% and 81% aa identical to human and mouse KIM-1 ECD, respectively. The only two reported ligands for KIM-1 are TIM-4 and the hepatitis A virus (9, 10). However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin (11). KIM-1 is found on CD4⁺ T cells and proximal renal tubular cells (4, 12). KIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 11). In particular, it induces IL-4 production, and requires the KIM-1 cytoplasmic tyrosine phosphorylation motif (12). Alternatively, KIM-1 activation of TIM-4 induces cell cycle arrest (13).

1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
5. Kuchroo, V.K. et al. (2006) Adv. Immunol. 91:227.
6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
8. Vaidya, V.S. et al. (2006) Am. J. Physiol. Renal Physiol. 290:F517.
9. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
10. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
11. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
12. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
13. Mesri, M. et al. (2006) Int. Immunol. 18:473.

Publications for TIM-1/KIM-1/HAVCR (3689-TM)(1)

Publication using 3689-TM

• JJ Sheu, PH Sung, CG Wallace, CC Yang, KH Chen, PL Shao, YC Chu, CR Huang, YL Chen, SF Ko, MS Lee, HK Yip Intravenous administration of iPS-MSCSPIONs mobilized into CKD parenchyma and effectively preserved residual renal function in CKD rat J. Cell. Mol. Med., 2020-02-15;0(0):. 2020-02-15 [PMID: 32061051] (IHC, Rat)

Bioinformatics

• Gene Symbol: Havcr1
• Uniprot:
  • O54947()